Antibacterial coatings on titanium surfaces: a comparison study between in vitro single-species and multispecies biofilm
Autor/a
Godoy-Gallardo, Maria
Wang, Zhejun
Shen, Ya
Gil Mur, Francisco Javier
Rodríguez Rius, Daniel
Haapasale, Markus
Fecha de publicación
2015ISSN
1944-8244
Resumen
Dental plaque is a biofilm that causes dental caries, gingivitis, and periodontitis. Most of the studies in antibacterial coatings have been conducted by in vitro single-species biofilm formation, but oral biofilm involves more than 700 different bacterial species that are able to interact. Therefore, new studies are focused on in vitro multispecies biofilm models that mimic in vivo biofilms. The aim of the present work was to study different antibacterial coatings onto titanium surfaces and evaluate the in vitro antimicrobial properties of the surfaces on two different bacterial species and an oral biofilm. The lactate dehydrogenase assay determined that treated samples did not affect fibroblast viability. In addition, the viability of microorganisms on modified samples was evaluated by a LIVE/DEAD BacLight bacterial viability kit. Although a decrease in viable bacteria onto treated samples was obtained, the results showed differences in effectiveness when single-biofilm and oral plaque were tested. It confirms, as we expected, the distinct sensitivities that bacterial strains have. Thus, this multispecies biofilms model holds a great potential to assess antibacterial properties onto samples for dental purposes.
Tipo de documento
Artículo
Versión del documento
Versión aceptada
Lengua
English
Materias (CDU)
616.3 - Patología del aparato digestivo. Odontología
Palabras clave
Biofilms
Pèptids i proteïnes
Bacteris
Titani
Plata
Biofilms
Péptidos y proteínas
Bacterias
Titanio
Plata
Biofilms
Peptides and proteins
Bacteria
Titanium
Silver
Páginas
10
Publicado por
American Chemical Society Publications
Colección
7; 10
Publicado en
ACS applied materials and interfaces
Citación
Godoy Gallardo, María; Wang, Zhejun; Shen, Ya [et al.]. Antibacterial coatings on titanium surfaces: A comparison study between in vitro single-species and multispecies biofilm. ACS applied materials and interfaces, 2015, 10, p. 5992-6001. Disponible en: <https://pubs.acs.org/doi/10.1021/acsami.5b00402>. Fecha de acceso: 12 nov. 2021. DOI: 10.1021/acsami.5b00402
Número del acuerdo de la subvención
info:eu-repo/grantAgreement/MINECO/MAT2009-12547
info:eu-repo/grantAgreement/MINECO/MAT2012-30706
Este ítem aparece en la(s) siguiente(s) colección(ones)
- Odontologia [242]
Derechos
© 2015 American Chemical Society