Antibacterial coatings on titanium surfaces: a comparison study between in vitro single-species and multispecies biofilm
Author
Godoy-Gallardo, Maria
Wang, Zhejun
Shen, Ya
Gil Mur, Francisco Javier
Rodríguez Rius, Daniel
Haapasale, Markus
Publication date
2015ISSN
1944-8244
Abstract
Dental plaque is a biofilm that causes dental caries, gingivitis, and periodontitis. Most of the studies in antibacterial coatings have been conducted by in vitro single-species biofilm formation, but oral biofilm involves more than 700 different bacterial species that are able to interact. Therefore, new studies are focused on in vitro multispecies biofilm models that mimic in vivo biofilms. The aim of the present work was to study different antibacterial coatings onto titanium surfaces and evaluate the in vitro antimicrobial properties of the surfaces on two different bacterial species and an oral biofilm. The lactate dehydrogenase assay determined that treated samples did not affect fibroblast viability. In addition, the viability of microorganisms on modified samples was evaluated by a LIVE/DEAD BacLight bacterial viability kit. Although a decrease in viable bacteria onto treated samples was obtained, the results showed differences in effectiveness when single-biofilm and oral plaque were tested. It confirms, as we expected, the distinct sensitivities that bacterial strains have. Thus, this multispecies biofilms model holds a great potential to assess antibacterial properties onto samples for dental purposes.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Biofilms
Pèptids i proteïnes
Bacteris
Titani
Plata
Biofilms
Péptidos y proteínas
Bacterias
Titanio
Plata
Biofilms
Peptides and proteins
Bacteria
Titanium
Silver
Pages
10
Publisher
American Chemical Society Publications
Collection
7; 10
Is part of
ACS applied materials and interfaces
Citation
Godoy Gallardo, María; Wang, Zhejun; Shen, Ya [et al.]. Antibacterial coatings on titanium surfaces: A comparison study between in vitro single-species and multispecies biofilm. ACS applied materials and interfaces, 2015, 10, p. 5992-6001. Disponible en: <https://pubs.acs.org/doi/10.1021/acsami.5b00402>. Fecha de acceso: 12 nov. 2021. DOI: 10.1021/acsami.5b00402
Grant agreement number
info:eu-repo/grantAgreement/MINECO/MAT2009-12547
info:eu-repo/grantAgreement/MINECO/MAT2012-30706
This item appears in the following Collection(s)
- Odontologia [242]
Rights
© 2015 American Chemical Society