Functionalization of CoCr surfaces with cell adhesive peptides to promote HUVECs adhesion and proliferation
Autor/a
Castellanos, Maria Isabel
Mas-Moruno, Carlos
Grau, Anna
Serra-Picamal, Xavier
Trepat, Xavier
Albericio Palomera, Fernando
Joner, Michael
Gil Mur, Francisco Javier
Ginebra, Maria-Pau
PEGUEROLES, MARTA
Fecha de publicación
2017ISSN
0169-4332
Resumen
Biomimetic surface modification with peptides that have specific cell-binding moieties is a promising approach to improve endothelialization of metal-based stents. In this study, we functionalized CoCr surfaces with RGDS, REDV, YIGSR peptides and their combinations to promote endothelial cells (ECs) adhesion and proliferation. An extensive characterization of the functionalized surfaces was performed by XPS analysis, surface charge and quartz crystal microbalance with dissipation monitoring (QCM-D), which demonstrated the successful immobilization of the peptides to the surface. Cell studies demonstrated that the covalent functionalization of CoCr surfaces with an equimolar combination of RGDS and YIGSR represents the most powerful strategy to enhance the early stages of ECs adhesion and proliferation, indicating a positive synergistic effect between the two peptide motifs. Although these peptide sequences slightly increased smooth muscle cells (SMCs) adhesion, these values were ten times lower than those observed for ECs. The combination of RGDS with the REDV sequence did not show synergistic effects in promoting the adhesion or proliferation of ECs. The strategy presented in this study holds great potential to overcome clinical limitations of current metal stents by enhancing their capacity to support surface endothelialization.
Tipo de documento
Artículo
Versión del documento
Versión aceptada
Lengua
English
Materias (CDU)
616.3 - Patología del aparato digestivo. Odontología
Palabras clave
Aliatge CoCr
Funcionalització superficial
Pèptids adhesius cel·lulars
Proliferació HUVEC
Endotelialització
Adhesió SMC
Aleación de CoCr
Funcionalización de superficie
Péptidos adhesivos celulares
Proliferación de HUVEC
Endotelización
Adherencia de SMC
CoCr alloy
Surface functionalization
Cell adhesive peptides
HUVEC proliferation
Endothelialization
SMCs adhesion
Páginas
37
Publicado por
Elsevier
Colección
393;
Publicado en
Applied Surface Science
Citación
Castellanos, Maria Isabel; Mas Moruno, Carlos; Grau, Anna [et al.]. Functionalization of CoCr surfaces with cell adhesive peptides to promote HUVECs adhesion and proliferation. Applied Surface Science, 2017, 393, p. 82-92. Disponible en: <https://www.sciencedirect.com/science/article/abs/pii/S0169433216319857?via%3Dihub>. Fecha de acceso: 11 nov. 2021. DOI: 10.1016/j.apsusc.2016.09.107
Número del acuerdo de la subvención
info:eu-repo/grantAgreement/MINECO/MAT2012-30706
info:eu-repo/grantAgreement/EC/FP7/321985
Este ítem aparece en la(s) siguiente(s) colección(ones)
- Odontologia [245]
Derechos
© 2016 Elsevier B.V. All rights reserved.