Citric acid passivation of titanium dental implants for minimizing bacterial colonization impact
Author
Punset fuste, Miquel
Vilarrasa, Javi
Nart Molina, José
Manero, José María
Padrós, Roberto
Pérez Antoñanzas, Roman
Gil Mur, Francisco Javier
Publication date
2021ISSN
2079-6412
Abstract
Surface topography and physical-chemical properties usually play a key-role in both osseointegration improvement and bacterial colonization reduction over the surface of dental implants. The aim of this study is to compare the chemical and bacteriological behavior of two different acid passivation surface treatments on titanium c.p. grade 3 used for dental implant manufacturing. Surface roughness was evaluated using White Light Interferometry (WLI) in order to determine different roughness parameters such as average roughness (Sa), the spacing parameter (Sm) and the hybrid parameter of surface index area (SIA). Contact angle (CA) and surface free energy (SFE) were evaluated in order to establish the surface wettability of the different groups of samples. Titanium ion-release from the different samples was also been analyzed in Hank’s solution medium at 37 °C by using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) at different immersion times. Bacterial viability adhesion assays were done using S. sanguinis (CECT 480, Spain) as a bacterial strain model of primary colonizer in oral biofilm formation. The bacteria attachment and morphology on Ti surfaces were determined using a live/dead staining method after 4 h of incubation and further analyzed by scanning electron microscope (SEM). Acid passivation surface treatments produced a statistically-significant (p < 0.05) roughness increase in all the evaluated parameters (Sa, Sm, SAI). The treatment with citric acid decreased the static contact angle (CA) and caused an increase in surface free energy (SFE) with a high polarization and oxidizing character. These physical-chemical surface characteristics obtained by means of citric acid passivation caused the bactericidal behavior as it has been proved in bacterial studies.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Passivació
Implants dentals
Alliberament d'ions
Titani
Gruix d'òxid
Rugositat superficial
Angle de contacte estàtic
Energia lliure de superfície
Adhesió bacteriana
Passivació de l'àcid cítric
Àcid cítric
Pasivación
Implantes dentales
Liberación de iones
Titanio
Espesor de óxido
Rugosidad superficial
Ángulo de contacto estático
Energía libre de superficie
Adhesión bacteriana
Pasivación del ácido cítrico
Ácido cítrico
Passivation
Dental implants
Release of ions
Titanium
Oxide thickness
Surface roughness
Static contact angle
Surface free energy
Bacterial adhesion
Passivation of citric acid
Citric acid
Pages
13
Publisher
MDPI
Collection
11;2
Is part of
Coatings
Citation
Punset, Miquel; Vilarrasa, Javi; Nart Molina, José [et al.]. Citric acid passivation of titanium dental implants for minimizing bacterial colonization impact. Coatings, 2021, 11(2), 214. Disponible en: <https://www.mdpi.com/2079-6412/11/2/214>. Fecha de acceso: 7 jun. 2021. DOI: 10.3390/coatings11020214
Grant agreement number
info:eu-repo/grantAgreement/MINECO/RTI2018-098075-B-C21
info:eu-repo/grantAgreement/MINECO/RTI2018-098075-BC22
This item appears in the following Collection(s)
- Odontologia [222]
Rights
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/