Dynamic force spectroscopy of DNA hairpins: I. Force kinetics and free energy landscapes
Author
Mossa, A.
Manosas, M.
Forns, N.
Huguet Casades, Josep Maria
Ritort, F.
Publication date
2009ISSN
1742-5468
Abstract
We investigate the thermodynamics and kinetics of DNA hairpins that fold/unfold under the action of applied mechanical force. We introduce the concept of the molecular free energy landscape and derive simplified expressions for the force dependent Kramers–Bell rates. To test the theory we have designed a specific DNA hairpin sequence that shows two-state cooperative folding under mechanical tension and carried out pulling experiments using optical tweezers. We show how we can determine the parameters that characterize the molecular free energy landscape of such sequences from rupture force kinetic studies. Finally we combine such kinetic studies with experimental investigations of the Crooks fluctuation relation to derive the free energy of formation of the hairpin at zero force.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
57 - Biological sciences in general
Keywords
DNA
ADN
Pages
Desconocido
Publisher
IOP Publishing
Collection
2009
Is part of
Journal of Statistical Mechanics: Theory and Experiment
Citation
Mossa, A.; Manosas, M.; Forns, N. [et al.]. Dynamic force spectroscopy of DNA hairpins: I. Force kinetics and free energy landscapes. Journal of Statistical Mechanics: Theory and Experiment, 2009, 2009, P02060. Disponible en: <https://iopscience.iop.org/article/10.1088/1742-5468/2009/02/P02060/pdf>. Fecha de acceso: 18 mar. 2024. DOI: 10.1088/1742-5468/2009/02/P02060
This item appears in the following Collection(s)
- Ciències Bàsiques [65]
Rights
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