dc.contributor.author | Tatullo, Marco | |
dc.contributor.author | Spagnuolo, Gianrico | |
dc.contributor.author | Codispoti, Bruna | |
dc.contributor.author | Zamparini, Fausto | |
dc.contributor.author | Zhang, Anqi | |
dc.contributor.author | Degli Esposti, Micaela | |
dc.contributor.author | Aparicio, Conrado | |
dc.contributor.author | Rengo, Carlo | |
dc.contributor.author | Nuzzolese, Manuel | |
dc.contributor.author | Manzoli, Lucia | |
dc.contributor.author | Fava, Fabio | |
dc.contributor.author | Prati, Carlo | |
dc.contributor.author | Fabbri, Paola | |
dc.contributor.author | Giovanna Gandolfi, Maria | |
dc.date.accessioned | 2023-04-19T09:40:54Z | |
dc.date.available | 2023-04-19T09:40:54Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Tatullo, Marco; Spagnuolo, Gianrico; Codispoti, Bruna [et al.]. PLA-based mineral-doped scaffolds seeded with human periapical cyst-derived MSCs: a promising tool for regenerative healing in dentistry. Materials, 2019, 12(4), 597. Disponible en: <https://www.mdpi.com/1996-1944/12/4/597>. Fecha de acceso: 19 abr. 2023. DOI: 10.3390/ma12040597 | ca |
dc.identifier.issn | 1996-1944 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/3668 | |
dc.description.abstract | Human periapical cyst mesenchymal stem cells (hPCy-MSCs) are a newly discovered cell population innovatively collected from inflammatory periapical cysts. The use of this biological waste guarantees a source of stem cells without any impact on the surrounding healthy tissues, presenting a valuable potential in tissue engineering and regenerative medicine applications. In the present study, hPCy-MSCs were collected, isolated, and seeded on three experimental mineral-doped porous scaffolds produced by the thermally-induced phase-separation (TIPS) technique. Mineral-doped scaffolds, composed of polylactic acid (PLA), dicalcium phosphate dihydrate (DCPD), and/or hydraulic calcium silicate (CaSi), were produced by TIPS (PLA-10CaSi, PLA-5CaSi-5DCPD, PLA-10CaSi-10DCPD). Micro-CT analysis evaluated scaffolds micromorphology. Collected hPCy-MSCs, characterized by cytofluorimetry, were seeded on the scaffolds and tested for cell proliferation, cells viability, and gene expression for osteogenic and odontogenic differentiation (DMP-1, OSC, RUNX-2, HPRT). Micro-CT revealed an interconnected highly porous structure for all the scaffolds, similar total porosity with 99% open pores. Pore wall thickness increased with the percentage of CaSi and DCPD. Cells seeded on mineral-doped scaffolds showed a superior proliferation compared to pure PLA scaffolds (control), particularly on PLA-10CaSi-10DCPD at day 12. A higher number of non-viable (red stained) cells was observable on PLA scaffolds at days 14 and 21. DMP-1 expression increased in hPCy-MSCs cultured on all mineral-doped scaffolds, in particular on PLA-5CaSi-5DCPD and PLA-10CaSi-10DCPD. In conclusion, the innovative combination of experimental scaffolds colonized with autologous stem cells from periapical cyst represent a promising strategy for regenerative healing of periapical and alveolar bone. | en |
dc.format.extent | 17 | ca |
dc.language.iso | eng | ca |
dc.publisher | MDPI | ca |
dc.relation.ispartof | Materials | ca |
dc.relation.ispartofseries | 12;4 | |
dc.relation.uri | https://www.mdpi.com/1996-1944/12/4/597 | ca |
dc.rights | © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject.other | Bastides a base d'àcid polilàctic (PLA) | ca |
dc.subject.other | Bastides que contenen silicat de calci | ca |
dc.subject.other | Bastides bioactives | ca |
dc.subject.other | Bastides poroses | ca |
dc.subject.other | Cèl·lules mare mesenquimals de quist periapical humà (hPCy-MSCs) | ca |
dc.subject.other | Odontologia regenerativa | ca |
dc.subject.other | Tècnica de separació de fases induïda tèrmicament (TIPS) | ca |
dc.subject.other | Armazones a base de ácido poliláctico (PLA) | es |
dc.subject.other | Armazones que contienen silicato de calcio | es |
dc.subject.other | Andamios bioactivos | es |
dc.subject.other | Andamios porosos | es |
dc.subject.other | Células madre mesenquimales de quistes periapicales humanos (hPCy-MSC) | es |
dc.subject.other | Odontología regenerativa | es |
dc.subject.other | Técnica de separación de fases inducida térmicamente (TIPS) | es |
dc.subject.other | Polylactic acid (PLA)-based scaffolds | en |
dc.subject.other | Calcium silicate-containing scaffolds | en |
dc.subject.other | Bioactive scaffolds | en |
dc.subject.other | Porous scaffolds | en |
dc.subject.other | Human periapical cyst mesenchymal stem cells (hPCy-MSCs) | en |
dc.subject.other | Regenerative dentistry | en |
dc.subject.other | Thermally induced phase separation technique (TIPS) | en |
dc.title | PLA-based mineral-doped scaffolds seeded with human periapical cyst-derived MSCs: a promising tool for regenerative healing in dentistry | en |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 616.3 | ca |
dc.identifier.doi | https://dx.doi.org/10.3390/ma12040597 | ca |