PLA-based mineral-doped scaffolds seeded with human periapical cyst-derived MSCs: a promising tool for regenerative healing in dentistry
Visualitza/Obre
Autor/a
Zhang, Anqi
Aparicio, Conrado
Rengo, Carlo
Nuzzolese, Manuel
Manzoli, Lucia
Fava, Fabio
Prati, Carlo
Fabbri, Paola
Giovanna Gandolfi, Maria
Data de publicació
2019ISSN
1996-1944
Resum
Human periapical cyst mesenchymal stem cells (hPCy-MSCs) are a newly discovered cell population innovatively collected from inflammatory periapical cysts. The use of this biological waste guarantees a source of stem cells without any impact on the surrounding healthy tissues, presenting a valuable potential in tissue engineering and regenerative medicine applications. In the present study, hPCy-MSCs were collected, isolated, and seeded on three experimental mineral-doped porous scaffolds produced by the thermally-induced phase-separation (TIPS) technique. Mineral-doped scaffolds, composed of polylactic acid (PLA), dicalcium phosphate dihydrate (DCPD), and/or hydraulic calcium silicate (CaSi), were produced by TIPS (PLA-10CaSi, PLA-5CaSi-5DCPD, PLA-10CaSi-10DCPD). Micro-CT analysis evaluated scaffolds micromorphology. Collected hPCy-MSCs, characterized by cytofluorimetry, were seeded on the scaffolds and tested for cell proliferation, cells viability, and gene expression for osteogenic and odontogenic differentiation (DMP-1, OSC, RUNX-2, HPRT). Micro-CT revealed an interconnected highly porous structure for all the scaffolds, similar total porosity with 99% open pores. Pore wall thickness increased with the percentage of CaSi and DCPD. Cells seeded on mineral-doped scaffolds showed a superior proliferation compared to pure PLA scaffolds (control), particularly on PLA-10CaSi-10DCPD at day 12. A higher number of non-viable (red stained) cells was observable on PLA scaffolds at days 14 and 21. DMP-1 expression increased in hPCy-MSCs cultured on all mineral-doped scaffolds, in particular on PLA-5CaSi-5DCPD and PLA-10CaSi-10DCPD. In conclusion, the innovative combination of experimental scaffolds colonized with autologous stem cells from periapical cyst represent a promising strategy for regenerative healing of periapical and alveolar bone.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
616.3 - Patologia de l'aparell digestiu. Odontologia
Paraules clau
Bastides a base d'àcid polilàctic (PLA)
Bastides que contenen silicat de calci
Bastides bioactives
Bastides poroses
Cèl·lules mare mesenquimals de quist periapical humà (hPCy-MSCs)
Odontologia regenerativa
Tècnica de separació de fases induïda tèrmicament (TIPS)
Armazones a base de ácido poliláctico (PLA)
Armazones que contienen silicato de calcio
Andamios bioactivos
Andamios porosos
Células madre mesenquimales de quistes periapicales humanos (hPCy-MSC)
Odontología regenerativa
Técnica de separación de fases inducida térmicamente (TIPS)
Polylactic acid (PLA)-based scaffolds
Calcium silicate-containing scaffolds
Bioactive scaffolds
Porous scaffolds
Human periapical cyst mesenchymal stem cells (hPCy-MSCs)
Regenerative dentistry
Thermally induced phase separation technique (TIPS)
Pàgines
17
Publicat per
MDPI
Col·lecció
12;4
Publicat a
Materials
Citació
Tatullo, Marco; Spagnuolo, Gianrico; Codispoti, Bruna [et al.]. PLA-based mineral-doped scaffolds seeded with human periapical cyst-derived MSCs: a promising tool for regenerative healing in dentistry. Materials, 2019, 12(4), 597. Disponible en: <https://www.mdpi.com/1996-1944/12/4/597>. Fecha de acceso: 19 abr. 2023. DOI: 10.3390/ma12040597
Enllaç al document relacionat
Aquest element apareix en la col·lecció o col·leccions següent(s)
- Odontologia [228]
Drets
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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