Human whole blood interactions with craniomaxillofacial reconstruction materials: exploring In vitro the role of blood cascades and leukocytes in early healing events
Visualitza/Obre
Autor/a
Anand, Vivek
Gallinetti, Sara
Data de publicació
2023ISSN
2079-4983
Resum
The present study investigated early interactions between three alloplastic materials (calcium phosphate (CaP), titanium alloy (Ti), and polyetheretherketone (PEEK) with human whole blood using an established in vitro slide chamber model. After 60 min of contact with blood, coagulation (thrombin–antithrombin complexes, TAT) was initiated on all test materials (Ti > PEEK > CaP), with a significant increase only for Ti. All materials showed increased contact activation, with the KK–AT complex significantly increasing for CaP (p < 0.001), Ti (p < 0.01), and PEEK (p < 0.01) while only CaP demonstrated a notable rise in KK-C1INH production (p < 0.01). The complement system had significant activation across all materials, with CaP (p < 0.0001, p < 0.0001) generating the most pronounced levels of C3a and sC5b-9, followed by Ti (p < 0.001, p < 0.001) and lastly, PEEK (p < 0.001, p < 0.01). This activation correlated with leukocyte stimulation, particularly myeloperoxidase release. Consequently, the complement system may assume a more significant role in the early stages post implantation in response to CaP materials than previously recognized. Activation of the complement system and the inevitable activation of leukocytes might provide a more favorable environment for tissue remodeling and repair than has been traditionally acknowledged. While these findings are limited to the early blood response, complement and leukocyte activation suggest improved healing outcomes, which may impact long-term clinical outcomes.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
616.3 - Patologia de l'aparell digestiu. Odontologia
Paraules clau
Biomaterials
Sang sencera humana
Coagulació
Complement
Fosfat de calci
Biomateriales
Sangre entera humana
Coagulación
Complementar
Fosfato de calcio
Biomaterials
Human whole blood
Coagulation
Complement
Calcium phosphate
Pàgines
17
Publicat per
MDPI
Col·lecció
14; 7
Publicat a
Journal of Functional Biomaterials
Citació
Lopes, Viviana; Birgersson, Ulrik; Anand, Vivek [et al.]. Human whole blood interactions with craniomaxillofacial reconstruction materials: exploring In vitro the role of blood cascades and leukocytes in early healing events. Journal of Functional Biomaterials, 2023, 14(7), 361. Disponible en: <https://www.mdpi.com/2079-4983/14/7/361>. Fecha de acceso: 14 nov. 2023. DOI: 10.3390/jfb14070361.
Aquest element apareix en la col·lecció o col·leccions següent(s)
- Odontologia [239]
Drets
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Excepte que s'indiqui una altra cosa, la llicència de l'ítem es descriu com https://creativecommons.org/licenses/by/4.0/