Relevant aspects of titanium topography for osteoblastic adhesion and inhibition of bacterial colonization
Autor/a
Rodriguez-González, Raquel
Monsalve-Guil, Loreto
Jimenez-Guerra, Alvaro
Nuñez-Marquez, Enrique
Ortiz-Garcia, Ivan
Fecha de publicación
2023ISSN
1996-1944
Resumen
The influence of the surface topography of dental implants has been studied to optimize titanium surfaces in order to improve osseointegration. Different techniques can be used to obtain rough titanium, however, their effect on wettability, surface energy, as well as bacterial and cell adhesion and differentiation has not been studied deeply. Two-hundred disks made of grade 4 titanium were subjected to different treatments: machined titanium (MACH), acid-attacked titanium (AE), titanium sprayed with abrasive alumina particles under pressure (GBLAST), and titanium that has been treated with GBLAST and then subjected to AE (GBLAST + AE). The roughness of the different treatments was determined by confocal microscopy, and the wettability was determined by the sessile drop technique; then, the surface energy of each treatment was calculated. Osteoblast-like cells (SaOs-2) were cultured, and alkaline phosphatase was determined using a colorimetric test. Likewise, bacterial strains S. gordonii, S. oralis, A. viscosus, and E. faecalis were cultured, and proliferation on the different surfaces was determined. It could be observed that the roughness of the GBLAST and GBLAS + AE was higher, at 1.99 and 2.13 μm of Ra, with respect to the AE and MACH samples, which were 0.35 and 0.20 μm, respectively. The abrasive treated surfaces showed lower hydrophilicity but lower surface energy. Significant differences could be seen at 21 days between SaOS-2 osteoblastic cell adhesion for the blasted ones and higher osteocalcin levels. However, no significant differences in terms of bacterial proliferation were observed between the four surfaces studied, demonstrating the insensitivity of bacteria to topography. These results may help in the search for the best topographies for osteoblast behavior and for the inhibition of bacterial colonization.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
616.3 - Patología del aparato digestivo. Odontología
Palabras clave
Titani
Rugositat
Topografia
Osteoblasts
Bacteris
Periimplantitis
Titanio
Aspereza
Topografía
Osteoblastos
Bacterias
Periimplantitis
Titanium
Roughness
Topography
Osteoblasts
Bacteria
Periimplantitis
Páginas
13
Publicado por
MDPI
Colección
16
Publicado en
Materials
Citación
Rodriguez-González, Raquel; Monsalve-Guil, Loreto; Jimenez-Guerra, Alvaro [et al.]. Relevant aspects of titanium topography for osteoblastic adhesion and inhibition of bacterial colonization. Materials, 2023, 16(9), 3553. Disponible en: <https://www.mdpi.com/1996-1944/16/9/3553>. Fecha de acceso: 9 jun. 2023. DOI: 10.3390/ma16093553
Enlace al documento relacionado
Este ítem aparece en la(s) siguiente(s) colección(ones)
- Odontologia [245]
Derechos
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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