Surface immobilization chemistry of a laminin-derived peptide affects keratinocyte activity
Publication date
2020ISSN
2079-6412
Abstract
Many chemical routes have been proposed to immobilize peptides on biomedical device surfaces, and in particular, on dental implants to prevent peri-implantitis. While a number of factors affect peptide immobilization quality, an easily controllable factor is the chemistry used to immobilize peptides. These factors affect peptide chemoselectivity, orientation, etc., and ultimately control biological activity. Using many different physical and chemical routes for peptide coatings, previous research has intensely focused on immobilizing antimicrobial elements on dental implants to reduce infection rates. Alternatively, our strategy here is different and focused on promoting formation of a long-lasting biological seal between the soft tissue and the implant surface through transmembrane, cell adhesion structures called hemidesmosomes. For that purpose, we used a laminin-derived call adhesion peptide. However, the effect of different immobilization chemistries on cell adhesion peptide activity is vastly unexplored but likely critical. Here, we compared the physiochemical properties and biological responses of a hemidesmosome promoting peptide immobilized using silanization and copper-free click chemistry as a model system for cell adhesion peptides. Successful immobilization was confirmed with water contact angle and X-ray photoelectron spectroscopy. Peptide coatings were retained through 73 days of incubation in artificial saliva. Interestingly, the non-chemoselective immobilization route, silanization, resulted in significantly higher proliferation and hemidesmosome formation in oral keratinocytes compared to chemoselective click chemistry. Our results highlight that the most effective immobilization chemistry for optimal peptide activity is dependent on the specific system (substrate/peptide/cell/biological activity) under study. Overall, a better understanding of the effects immobilization chemistries have on cell adhesion peptide activity may lead to more efficacious coatings for biomedical devices.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Superfícies
Pèptids
Immobilització
Queratinòcits
Hemidesmosomes
Implants dentals
Periimplantitis
Laminina
Superficies
Péptidos
Inmovilización
Queratinocitos
Hemidesmosomas
Implantes dentales
Periimplantitis
Laminina
Surfaces
Peptides
Immobilization
Keratinocytes
Hemidesmosomes
Dental implants
Peri-implantitis
Laminin
Pages
14
Publisher
MDPI
Collection
10; 6
Is part of
Coatings
Citation
Fischer, Nicholas; He, Jiahe; Aparicio, Conrado. Surface immobilization chemistry of a laminin-derived peptide affects keratinocyte activity. Coatings, 2020, 10(6), 560. Disponible en: <https://www.mdpi.com/2079-6412/10/6/560>. Fecha de acceso: 19 abr. 2023. DOI: 10.3390/coatings10060560
Link to the related item
This item appears in the following Collection(s)
- Odontologia [245]
Rights
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by/4.0/