On-growth and In-growth osseointegration enhancement in PM porous Ti-scaffolds by two different bioactivation strategies: alkali thermochemical treatment and RGD Ppeptide coating
Author
Rappe, Katrin Steffanie
Ortiz, Monica
Punset fuste, Miquel
Molmeneu, Meritxell
Barba, Albert
Mas-Moruno, Carlos
Guillem-Marti, Jordi
Caparrós, Cristina
Ruperez, Elisa
Calero, José
Manzanares Céspedes, Maria Cristina
Franch, Jordi
Publication date
2022ISSN
1422-0067
Abstract
A lack of primary stability and osteointegration in metallic implants may result in implant loosening and failure. Adding porosity to metallic implants reduces the stress shielding effect and improves implant performance, allowing the surrounding bone tissue to grow into the scaffold. However, a bioactive surface is needed to stimulate implant osteointegration and improve mechanical stability. In this study, porous titanium implants were produced via powder sintering to create different porous diameters and open interconnectivity. Two strategies were used to generate a bioactive surface on the metallic foams: (1) an inorganic alkali thermochemical treatment, (2) grafting a cell adhesive tripeptide (RGD). RGD peptides exhibit an affinity for integrins expressed by osteoblasts, and have been reported to improve osteoblast adhesion, whereas the thermochemical treatment is known to improve titanium implant osseointegration upon implantation. Bioactivated scaffolds and control samples were implanted into the tibiae of rabbits to analyze the effect of these two strategies in vivo regarding bone tissue regeneration through interconnected porosity. Histomorphometric evaluation was performed at 4 and 12 weeks after implantation. Bone-to-implant contact (BIC) and bone in-growth and on-growth were evaluated in different regions of interest (ROIs) inside and outside the implant. The results of this study show that after a long-term postoperative period, the RGD-coated samples presented higher quantification values of quantified newly formed bone tissue in the implant’s outer area. However, the total analyzed bone in-growth was observed to be slightly greater in the scaffolds treated with alkali thermochemical treatment. These results suggest that both strategies contribute to enhancing porous metallic implant stability and osteointegration, and a combination of both strategies might be worth pursuing.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Escumes de titani
Osteointegració
Tractament termoquímic
Pèptid RGD
En viu implantació
Avaluació histomorfomètrica
Creixement ossi
Espumas de titanio
Osteointegración
Tratamiento termoquímico
Péptido RGD
En vivo implantación
Evaluación histomorfométrica
Crecimiento óseo
Titanium foams
Osseointegration
Thermochemical treatment
RGD peptide
In vivo implantation
Histomorphometric evaluation
Bone on-growth
Pages
26
Publisher
MDPI
Collection
23;3
Is part of
International Journal of Molecular Sciences
Citation
Rappe, Katrin Steffanie; Ortiz, Monica; Punset Fuste, Miquel [et al.]. On-growth and In-growth osseointegration enhancement in PM porous Ti-scaffolds by two different bioactivation strategies: alkali thermochemical treatment and RGD Ppeptide coating. International Journal of Molecular Sciences, 2022, 23(3), 1750. Disponible en: <https://www.mdpi.com/1422-0067/23/3/1750>. Fecha de acceso: 24 feb. 2023. DOI: 10.3390/ijms23031750
Grant agreement number
info:eu-repo/grantAgreement/ES/2PE/RTI2018-098075-B-C21
info:eu-repo/grantAgreement/ES/2PE/RTI2018-098075-B-C22
info:eu-repo/grantAgreement/ES/2PE/PID2020-114019RB-I00
Link to the related item
This item appears in the following Collection(s)
- Odontologia [228]
Rights
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/