Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis
Author
Castellanos, Maria Isabel
Guillem-Marti, Jordi
Mas-Moruno, Carlos
DIAZ-RICART, MARIBEL
Escolar Albaladejo, Ginés
Ginebra, Maria-Pau
Gil Mur, Francisco Javier
PEGUEROLES, MARTA
Manero, José María
Publication date
2017ISSN
1549-3296
Abstract
Immobilization of bioactive peptide sequences on CoCr surfaces is an effective route to improve endothelialization, which is of great interest for cardiovascular stents. In this work, we explored the effect of physical and covalent immoblization of RGDS, YIGSR and their equimolar combination peptides on endothelial cells (EC) and smooth muscle cell (SMC) adhesion and on thrombogenicity. We extensively investigated using RT-qPCR, the expression by ECs cultured on functionalised CoCr surfaces of different genes. Genes relevant for adhesion (ICAM-1 and VCAM-1), vascularization (VEGFA, VEGFR-1 and VEGFR-2) and anti-thrombogenicity (tPA and eNOS) were over-expressed in the ECs grown to covalently functionalized CoCr surfaces compared to physisorbed and control surfaces. Pro-thrombogenic genes expression (PAI-1 and vWF) decreased over time. Cell co-cultures of ECs/SMCs found that functionalization increased the amount of adhered ECs onto modified surfaces compared to plain CoCr, independently of the used peptide and the strategy of immobilization. SMCs adhered less compared to ECs in all surfaces. All studied peptides showed a lower platelet cell adhesion compared to TCPS. Covalent functionalization of CoCr surfaces with an equimolar combination of RGDS and YIGSR represented prevailing strategy to enhance the early stages of ECs adhesion and proliferation, while preventing SMCs and platelet adhesion.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Pèptids adhesius cel·lulars
Aliatge de CoCr
Endotelització
Trombogènesi
Restenosi
Péptidos adhesivos celulares
Aleación de CoCr
Endotelización
Trombogénesis
Restenosis
Cell adhesive peptides
CoCr alloy
Endothelialization
Thrombogenesis
Restenosis
Pages
11
Publisher
John Wiley & Sons
Collection
105;4
Is part of
Journal of biomedical materials research. Part A
Citation
Castellanos, Maria Isabel; Guillem Martí, Jordi; Mas Moruno, Carlos [et al.]. Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis. Journal of biomedical materials research. Part A, 2017, 105(4), p. 973-983. Disponible en: <https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35988>. Fecha de acceso: 16 nov. 2021. DOI: 10.1002/jbm.a.35988
This item appears in the following Collection(s)
- Odontologia [228]
Rights
© 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 973–983, 2017.