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dc.contributor.authorBrotons de Los Reyes, Pedro
dc.contributor.authorPerez-Argüello, Amaresh
dc.contributor.authorLaunes, Cristian
dc.contributor.authorTorrents, Francesc
dc.contributor.authorSubirats, Maria Pilar
dc.contributor.authorSaucedo, Jesica
dc.contributor.authorClaverol, Joana
dc.contributor.authorGarcia-Garcia, Juan Jose
dc.contributor.authorRodas Font, Gil
dc.contributor.authorFumado, Vicky
dc.contributor.authorJordan, Iolanda
dc.contributor.authorGratacos, Eduard
dc.contributor.authorBassat, Quique
dc.contributor.authorMuñoz-Almagro, Carmen
dc.date.accessioned2021-10-06T15:50:17Z
dc.date.available2021-10-06T15:50:17Z
dc.date.issued2021
dc.identifier.citationBrotons de Los Reyes, Pedro; Perez-Argüello, Amaresh; Launes, Cristian [et al.]. Validation and implementation of a direct RT-qPCR method for rapid screening of SARS-CoV-2 infection by using non-invasive saliva samples. International Journal of Infectious Diseases, 2021, 110, p. 363-370. Disponible en: <https://www.sciencedirect.com/science/article/pii/S120197122100607X?via%3Dihub>. Fecha de acceso: 6 oct. 2021. DOI: 10.1016/j.ijid.2021.07.054ca
dc.identifier.issn1201-9712ca
dc.identifier.urihttp://hdl.handle.net/20.500.12328/2844
dc.description.abstractObjective: To validate and implement an optimized screening method for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA combining use of self-collected raw saliva samples, single-step heat-treated virus inactivation and RNA extraction, and direct RT-qPCR. Methods: This was a three-phase study conducted in Barcelona (Spain) during June to October, 2020. The three phases were (1) analytical validation against standard RT-qPCR in saliva samples; (2) diagnostic validation against standard RT-qPCR using paired saliva–nasopharyngeal samples obtained from asymptomatic teenagers and adults in a sports academy; and (3) pilot screening of asymptomatic health workers in a tertiary hospital. Results: In phase 1, the detection yield of the new method was comparable to that of standard RT-qPCR. In phase 2, the diagnostic sensitivity and specificity values in 303 self-collected saliva samples were 95.7% (95% confidence interval 79.0–99.2%) and 100.0% (95% confidence interval 98.6–100.0%), respectively. In phase 3, only 17 (0.6%) of the saliva samples self-collected by 2709 participants without supervision were invalid. The rapid analytical workflow with the new method (up to 384 batched samples could be processed in less than 2 hours) yielded 24 (0.9%) positive results in the remaining 2692 saliva samples. Paired nasopharyngeal specimens were all positive by standard RT-qPCR. Conclusions: Direct RT-qPCR on self-collected raw saliva is a simple, rapid, and accurate method with potential to be scaled up for enhanced SARS-CoV-2 community-wide screening.en
dc.format.extent8ca
dc.language.isoengca
dc.publisherElsevierca
dc.relation.ispartofInternational Journal of Infectious Diseasesca
dc.relation.ispartofseries110;
dc.rights© 2021 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.otherCOVID-19 (Malaltia)ca
dc.subject.otherPCRca
dc.subject.otherSalivaca
dc.subject.otherProjeccióca
dc.subject.otherVigilànciaca
dc.subject.otherCOVID-19es
dc.subject.otherPCRes
dc.subject.otherSalivaes
dc.subject.otherProyecciónes
dc.subject.otherVigilanciaes
dc.subject.otherCOVID-19en
dc.subject.otherPCRen
dc.subject.otherSalivaen
dc.subject.otherProjectionen
dc.subject.otherSurveillanceen
dc.titleValidation and implementation of a direct RT-qPCR method for rapid screening of SARS-CoV-2 infection by using non-invasive saliva samplesen
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc61ca
dc.identifier.doihttps://dx.doi.org/10.1016/j.ijid.2021.07.054ca


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© 2021 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc-nd/4.0/
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