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dc.contributor.authorPanda, Suchita
dc.contributor.authorEl khader, Ismail
dc.contributor.authorCasellas, Francesc
dc.contributor.authorLopez Vivancos, Josefa
dc.contributor.authorGarcía Cors, Montserrat
dc.contributor.authorSantiago, Alba
dc.contributor.authorCuenca, Silvia
dc.contributor.authorGuarner, Francisco
dc.contributor.authorManichanh, Chaysavanh
dc.date.accessioned2019-11-10T18:00:39Z
dc.date.available2019-11-10T18:00:39Z
dc.date.issued2014-04-18
dc.identifier.citationPanda, Suchita; El khader, Ismail; Casellas, Francesc López; Vivancos, Josefa; García Cors, Montserrat; Santiago, Alba; Cuenca, Silvia; Guarner, Francisco; Manichanh, Chaysavanh. Short-Term Effect of Antibiotics on Human Gut Microbiota. PLoS ONE, 2014, vol.9, núm 4. Disponible en: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0095476#references>. Fecha de acceso: 10 nov 2019. DOI: 10.1371/journal.pone.0095476ca
dc.identifier.issn1932-6203ca
dc.identifier.urihttp://hdl.handle.net/20.500.12328/1300
dc.description.abstractFrom birth onwards, the human gut microbiota rapidly increases in diversity and reaches an adult-like stage at three years of age. After this age, the composition may fluctuate in response to external factors such as antibiotics. Previous studies have shown that resilience is not complete months after cessation of the antibiotic intake. However, little is known about the short-term effects of antibiotic intake on the gut microbial community. Here we examined the load and composition of the fecal microbiota immediately after treatment in 21 patients, who received broad-spectrum antibiotics such as fluoroquinolones and β-lactams. A fecal sample was collected from all participants before treatment and one week after for microbial load and community composition analyses by quantitative PCR and pyrosequencing of the 16S rRNA gene, respectively. Fluoroquinolones and β-lactams significantly decreased microbial diversity by 25% and reduced the core phylogenetic microbiota from 29 to 12 taxa. However, at the phylum level, these antibiotics increased the Bacteroidetes/Firmicutes ratio (p = 0.0007, FDR = 0.002). At the species level, our findings unexpectedly revealed that both antibiotic types increased the proportion of several unknown taxa belonging to the Bacteroides genus, a Gram-negative group of bacteria (p = 0.0003, FDR<0.016). Furthermore, the average microbial load was affected by the treatment. Indeed, the β-lactams increased it significantly by two-fold (p = 0.04). The maintenance of or possible increase detected in microbial load and the selection of Gram-negative over Gram-positive bacteria breaks the idea generally held about the effect of broad-spectrum antibiotics on gut microbiota.ca
dc.format.extent7ca
dc.language.isoengca
dc.publisherPublic Library of Scienceca
dc.relation.ispartofPLoS ONEca
dc.relation.ispartofseries9;4
dc.rightsPLOS applies the Creative Commons Attribution Attribution 4.0 International (CC BY 4.0) license to articles and other works we publish.ca
dc.subject.otherIntestins--Microbiologiaca
dc.subject.otherAntibiòticsca
dc.subject.otherBactericidesca
dc.subject.otherToxines bacterianesca
dc.subject.otherIntestinos Microbiología
dc.subject.otherAntibióticos
dc.subject.otherBacteriología
dc.subject.otherToxinas
dc.subject.otherMicrobiology
dc.subject.otherAntibiotics
dc.subject.otherToxins--Analysis
dc.titleShort-term effect of antibiotics on human gut microbiotaca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.embargo.termscapca
dc.subject.udc61ca
dc.identifier.doihttps://dx.doi.org/10.1371/journal.pone.0095476ca


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