Improving signal/noise resolution in single-molecule experiments using molecular constructs with short handles
Autor/a
Forns, N.
de Lorenzo, S.
Manosas, M.
Hayashi, K.
Huguet Casades, Josep Maria
Ritort, F.
Fecha de publicación
2011ISSN
0006-3495
Resumen
We investigate unfolding/folding force kinetics in DNA hairpins exhibiting two and three states with newly designed short dsDNA handles (29 bp) using optical tweezers. We show how the higher stiffness of the molecular setup moderately enhances the signal/noise ratio (SNR) in hopping experiments as compared to conventional long-handled constructs (≅700 bp). The shorter construct results in a signal of higher SNR and slower folding/unfolding kinetics, thereby facilitating the detection of otherwise fast structural transitions. A novel analysis, as far as we are aware, of the elastic properties of the molecular setup, based on high-bandwidth measurements of force fluctuations along the folded branch, reveals that the highest SNR that can be achieved with short handles is potentially limited by the marked reduction of the effective persistence length and stretch modulus of the short linker complex.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
61 - Medicina
Palabras clave
ADN
Páginas
9
Publicado por
Elsevier
Colección
100; 7
Publicado en
Biophysical Journal
Citación
Forns, N.; de Lorenzo, S.; Manosas, M. [et al.]. Improving signal/noise resolution in single-molecule experiments using molecular constructs with short handles. Biophysical Journal, 2011, 100(7), p. 1765-1774. Disponible en: <https://www.sciencedirect.com/science/article/pii/S0006349511002013?via%3Dihub>. Fecha de acceso: 18 mar. 2024. DOI: 10.1016/j.bpj.2011.01.071
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Derechos
© 2011 Biophysical Society. Published by Elsevier Inc. All rights reserved.