Structural characterization of NORAD reveals a stabilizing role of spacers and two new repeat units
Autor/a
Chorostecki, Uciel
Saus, Ester
Gabaldón, Toni
Data de publicació
2021ISSN
2001-0370
Resum
Long non-coding RNAs (lncRNAs) can perform a variety of key cellular functions by interacting with proteins and other RNAs. Recent studies have shown that the functions of lncRNAS are largely mediated by their structures. However, our structural knowledge for most lncRNAS is limited to sequence-based computational predictions. Non-coding RNA activated by DNA damage (NORAD) is an atypical lncRNA due to its abundant expression and high sequence conservation. NORAD regulates genomic stability by interacting with proteins and microRNAs. Previous sequence-based characterization has identified a modular organization of NORAD composed of several NORAD repeat units (NRUs). These units comprise the protein-binding elements and are separated by regular spacers. Here, we experimentally determine for the first time the secondary structure of NORAD using the nextPARS approach. Our results suggest that the spacer regions provide structural stability to NRUs. Furthermore, we uncover two previously unreported NRUs, and determine the core structural motifs conserved across NRUs. Overall, these findings will help to elucidate the function and evolution of NORAD.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
English
Matèries (CDU)
5 - Ciències pures i naturals
Paraules clau
ARN llargs no codificants
NORAD
Estructura de l'ARN
ncRNAs
Long non-coding RNAs
NORAD
RNA structure
ncRNAs
Pàgines
9
Publicat per
Elsevier
Col·lecció
19
Publicat a
Computational and Structural Biotechnology Journal
Citació
Chorostecki, Uciel; Saus, Ester; Gabaldón, Toni. Structural characterization of NORAD reveals a stabilizing role of spacers and two new repeat units. Computational and Structural Biotechnology Journal, 2021, 19, p. 3245-3254. Disponible en: <https://www.sciencedirect.com/science/article/pii/S2001037021002269>. Fecha de acceso: 7 feb. 2024. DOI: 10.1016/j.csbj.2021.05.045
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