Prevention of posterior capsule opacification through intracapsular hydrogen peroxide or distilled water treatment in human donor tissue
Autor/a
D’Antin, Justin Christopher
Barraquer, Rafael I.
Tresserra, Francisco
Michael, Ralph
Fecha de publicación
2018ISSN
2045-2322
Resumen
In order to determine whether posterior capsule opacification after cataract surgery, could be delayed or inhibited through the application of hydrogen peroxide (H2O2) or distilled water (H2Od),we extracted lens capsules from 25 human donor eye globes. Samples were treated for 5 min with either 30 mM H2O2 or H2Od or used as controls, and cultured for one month, during which dark field and tilt illumination photos were taken. These were used to observe and quantify, time until cellular growth and confluence on the posterior capsule. After culture, histological sections were stained for H&E, α-SMA, Ki-67 and vimentin and evaluated. We prevented cellular growth in 50% of H2Od and 58% H2O2 of treated samples. The overall prevention of cell growth compared to cultured controls was significant for both treatments while there was no significant difference between them. In the cases where cellular growth was not prevented, both treatments significantly delay cellular growth. Until day 28 none of the treated samples of either type that had shown growth reached total confluence. All cultured controls reached total confluence before treated samples (median = day 11.5). Also, histologically, there was a clear morphological difference between cultured controls and treated samples.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
61 - Medicina
Palabras clave
Transició epitelial-mesenquimal
Models experimentals de malaltia
Transición epitelial-mesenquimatosa
Modelos experimentales de enfermedad
Epithelial–mesenchymal transition
Experimental models of disease
Páginas
10
Publicado por
Springer Nature
Colección
8
Publicado en
Scientific Reports
Citación
D’Antin, Justin Christopher; Barraquer, Rafael I.; Tresserra, Francisco [et al.]. Prevention of posterior capsule opacification through intracapsular hydrogen peroxide or distilled water treatment in human donor tissue. Scientific Reports, 2018, 8, 12739. Disponible en: <https://www.nature.com/articles/s41598-018-31178-y>. Fecha de acceso: 29 ene. 2024. DOI: 10.1038/s41598-018-31178-y
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