dc.contributor.author | Cabré, Gisela | |
dc.contributor.author | Garrido-Charles, Aida | |
dc.contributor.author | Moreno, Miquel | |
dc.contributor.author | Bosch, Miquel | |
dc.contributor.author | Porta-de-la-Riva, Montserrat | |
dc.contributor.author | Krieg, Michael | |
dc.contributor.author | Gascón-Moya, Marta | |
dc.contributor.author | Camarero, Núria | |
dc.contributor.author | Gelabert, Ricard | |
dc.contributor.author | Lluch, José M. | |
dc.contributor.author | Busqué, Félix | |
dc.contributor.author | Hernando, Jordi | |
dc.contributor.author | Gorostiza, Pau | |
dc.contributor.author | Alibés, Ramon | |
dc.date.accessioned | 2023-02-02T15:29:20Z | |
dc.date.available | 2023-02-02T15:29:20Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Cabré, Gisela; Garrido-Charles, Aida; Moreno, Miquel [et al]. Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation. Nature Communications, 2019, 10, 907. Disponible en: <https://www.nature.com/articles/s41467-019-08796-9>. Fecha de acceso: 2 feb. 2023. DOI: 10.1038/s41467-019-08796-9 | ca |
dc.identifier.issn | 2041-1723 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/3546 | |
dc.description.abstract | Manipulation of neuronal activity using two-photon excitation of azobenzene photoswitches with near-infrared light has been recently demonstrated, but their practical use in neuronal tissue to photostimulate individual neurons with three-dimensional precision has been hampered by firstly, the low efficacy and reliability of NIR-induced azobenzene photoisomerization compared to one-photon excitation, and secondly, the short cis state lifetime of the two-photon responsive azo switches. Here we report the rational design based on theoretical calculations and the synthesis of azobenzene photoswitches endowed with both high two-photon absorption cross section and slow thermal back-isomerization. These compounds provide optimized and sustained two-photon neuronal stimulation both in light-scattering brain tissue and in Caenorhabditis elegans nematodes, displaying photoresponse intensities that are comparable to those achieved under one-photon excitation. This finding opens the way to use both genetically targeted and pharmacologically selective azobenzene photoswitches to dissect intact neuronal circuits in three dimensions. | en |
dc.format.extent | 12 | ca |
dc.language.iso | eng | ca |
dc.publisher | Springer Nature | ca |
dc.relation.ispartof | Nature Communications | ca |
dc.relation.ispartofseries | 10 | |
dc.relation.uri | https://www.nature.com/articles/s41467-019-08796-9 | ca |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject.other | Canals de calci | ca |
dc.subject.other | Eines químiques | ca |
dc.subject.other | Fotobiologia | ca |
dc.subject.other | Canales de calcio | es |
dc.subject.other | Herramientas químicas | es |
dc.subject.other | Fotobiología | es |
dc.subject.other | Calcium channels | en |
dc.subject.other | Chemical tools | en |
dc.subject.other | Photobiology | en |
dc.title | Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation | en |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 6 | ca |
dc.identifier.doi | https://dx.doi.org/10.1038/s41467-019-08796-9 | ca |