Modulation of macrophage response by copper and magnesium ions in combination with low concentrations of dexamethasone
Autor/a
Díez-Tercero, Leire
Delgado, Luis M.
Antoñanzas Perez, Roman
Fecha de publicación
2022ISSN
2227-9059
Resumen
Macrophages have been deemed crucial for correct tissue regeneration, which is a complex process with multiple overlapping phases, including inflammation. Previous studies have suggested that divalent ions are promising cues that can induce an anti-inflammatory response, since they are stable cues that can be released from biomaterials. However, their immunomodulatory potential is limited in a pro-inflammatory environment. Therefore, we investigated whether copper and magnesium ions combined with low concentrations of the anti-inflammatory drug, dexamethasone (dex), could have a synergistic effect in macrophage, with or without pro-inflammatory stimulus, in terms of morphology, metabolic activity and gene expression. Our results showed that the combination of copper and dex strongly decreased the expression of pro-inflammatory markers, while the combination with magnesium upregulated the expression of IL-10. Moreover, in the presence of a pro-inflammatory stimulus, the combination of copper and dex induced a strong TNF-α response, suggesting an impairment of the anti-inflammatory actions of dex. The combination of magnesium and dex in the presence of a pro-inflammatory stimulus did not promote any improvement in comparison to dex alone. The results obtained in this study could be relevant for tissue engineering applications and in the design of platforms with a dual release of divalent ions and small molecules.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
English
Materias (CDU)
61 - Medicina
Palabras clave
Macròfags
Polarització
Ions
Coure
Magnesi
Dexametasona
Inflamació
Regeneració dels teixits
Macrófago
Polarización
Iones
Cobre
Magnesio
Dexametasona
Inflamación
Regeneración de tejidos
Macrophage
Polarization
Ions
Copper
Magnesium
Dexamethasone
Inflammation
Tissue regeneration
Páginas
16
Publicado por
MDPI
Colección
10; 4
Publicado en
Biomedicines
Citación
Díez-Tercero, Leire; Delgado, Luis M.; Antoñanzas Perez, Roman [et al.]. Modulation of macrophage response by copper and magnesium ions in combination with low concentrations of dexamethasone. Biomedicines, 2022, 10(4), 764. Disponible en: <https://www.mdpi.com/2227-9059/10/4/764>. Fecha de acceso: 19 abr. 2022. DOI: 10.3390/biomedicines10040764
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