dc.contributor.author | Sanchez-Fernandez, Mayka | |
dc.contributor.author | Galy, Bruno | |
dc.contributor.author | Dandekar, Thomas | |
dc.contributor.author | Bengert, Peter | |
dc.contributor.author | Vainshtein, Yevhen | |
dc.contributor.author | Stolte, Jens | |
dc.contributor.author | Muckenthaler, Martina | |
dc.contributor.author | Hentze, Matthias | |
dc.date.accessioned | 2022-01-17T15:58:52Z | |
dc.date.available | 2022-01-17T15:58:52Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Sanchez, Mayka; Galy, Bruno; Dandekar, Thomas [et al.]. Iron Regulation and the Cell Cycle: identification of an iron-responsive element in the 3′-untranslated region of human cell division cycle 14a mrna by a refined microarray-based screening strategy. The Journal of Biological Chemistry, 2006, 281(32), p. 22865-22874. Disponible en: <https://www.sciencedirect.com/science/article/pii/S0021925819476266?via%3Dihub>. Fecha de acceso: 17 ene. 2022. DOI: 10.1074/jbc.M603876200 | ca |
dc.identifier.issn | 0021-9258 | ca |
dc.identifier.uri | http://hdl.handle.net/20.500.12328/3070 | |
dc.description.abstract | Iron regulatory proteins (IRPs) 1 and 2 post-transcriptionally control mammalian iron homeostasis by binding to iron-responsive elements (IREs), conserved RNA stem-loop structures located in the 5′- or 3′-untranslated regions of genes involved in iron metabolism (e.g. FTH1, FTL, and TFRC). To identify novel IRE-containing mRNAs, we integrated biochemical, biocomputational, and microarray-based experimental approaches. IRP/IRE messenger ribonucleoproteins were immunoselected, and their mRNA composition was analyzed using an IronChip microarray enriched for genes predicted computationally to contain IRE-like motifs. Among different candidates, this report focuses on a novel IRE located in the 3′-untranslated region of the cell division cycle 14A mRNA. We show that this IRE motif efficiently binds both IRP1 and IRP2. Differential splicing of cell division cycle 14A produces IRE- and non-IRE-containing mRNA isoforms. Interestingly, only the expression of the IRE-containing mRNA isoforms is selectively increased by cellular iron deficiency. This work describes a new experimental strategy to explore the IRE/IRP regulatory network and uncovers a previously unrecognized regulatory link between iron metabolism and the cell cycle. | en |
dc.format.extent | 10 | ca |
dc.language.iso | eng | ca |
dc.publisher | Elsevier | ca |
dc.relation.ispartof | The Journal of Biological Chemistry | ca |
dc.relation.ispartofseries | 281;32 | |
dc.rights | Under a Creative Commons license. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject.other | Regulació del ferro | ca |
dc.subject.other | Cicle cel·lular | ca |
dc.subject.other | Proteïnes reguladores del ferro | ca |
dc.subject.other | Homeòstasi del ferro | ca |
dc.subject.other | Regulación del hierro | es |
dc.subject.other | Ciclo celular | es |
dc.subject.other | Proteínas reguladoras del hierro | es |
dc.subject.other | Homeostasis del hierro | es |
dc.subject.other | Iron regulation | en |
dc.subject.other | Cell cycle | en |
dc.subject.other | Iron-regulating proteins | en |
dc.subject.other | Iron homeostasis | en |
dc.title | Iron Regulation and the Cell Cycle: identification of an iron-responsive element in the 3′-untranslated region of human cell division cycle 14a mrna by a refined microarray-based screening strategy | ca |
dc.type | info:eu-repo/semantics/article | ca |
dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
dc.embargo.terms | cap | ca |
dc.subject.udc | 57 | ca |
dc.identifier.doi | https://dx.doi.org/10.1074/jbc.M603876200 | ca |