Iron Regulation and the Cell Cycle: identification of an iron-responsive element in the 3′-untranslated region of human cell division cycle 14a mrna by a refined microarray-based screening strategy
Visualitza/Obre
Autor/a
Sanchez-Fernandez, Mayka
Galy, Bruno
Dandekar, Thomas
Bengert, Peter
Vainshtein, Yevhen
Stolte, Jens
Muckenthaler, Martina
Hentze, Matthias
Data de publicació
2006ISSN
0021-9258
Resum
Iron regulatory proteins (IRPs) 1 and 2 post-transcriptionally control mammalian iron homeostasis by binding to iron-responsive elements (IREs), conserved RNA stem-loop structures located in the 5′- or 3′-untranslated regions of genes involved in iron metabolism (e.g. FTH1, FTL, and TFRC). To identify novel IRE-containing mRNAs, we integrated biochemical, biocomputational, and microarray-based experimental approaches. IRP/IRE messenger ribonucleoproteins were immunoselected, and their mRNA composition was analyzed using an IronChip microarray enriched for genes predicted computationally to contain IRE-like motifs. Among different candidates, this report focuses on a novel IRE located in the 3′-untranslated region of the cell division cycle 14A mRNA. We show that this IRE motif efficiently binds both IRP1 and IRP2. Differential splicing of cell division cycle 14A produces IRE- and non-IRE-containing mRNA isoforms. Interestingly, only the expression of the IRE-containing mRNA isoforms is selectively increased by cellular iron deficiency. This work describes a new experimental strategy to explore the IRE/IRP regulatory network and uncovers a previously unrecognized regulatory link between iron metabolism and the cell cycle.
Tipus de document
Article
Versió del document
Versió publicada
Llengua
Anglès
Matèries (CDU)
57 - Biologia
Paraules clau
Regulació del ferro
Cicle cel·lular
Proteïnes reguladores del ferro
Homeòstasi del ferro
Regulación del hierro
Ciclo celular
Proteínas reguladoras del hierro
Homeostasis del hierro
Iron regulation
Cell cycle
Iron-regulating proteins
Iron homeostasis
Pàgines
10
Publicat per
Elsevier
Col·lecció
281;32
Publicat a
The Journal of Biological Chemistry
Citació
Sanchez, Mayka; Galy, Bruno; Dandekar, Thomas [et al.]. Iron Regulation and the Cell Cycle: identification of an iron-responsive element in the 3′-untranslated region of human cell division cycle 14a mrna by a refined microarray-based screening strategy. The Journal of Biological Chemistry, 2006, 281(32), p. 22865-22874. Disponible en: <https://www.sciencedirect.com/science/article/pii/S0021925819476266?via%3Dihub>. Fecha de acceso: 17 ene. 2022. DOI: 10.1074/jbc.M603876200
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