Differential nasopharyngeal microbiota composition in children according to respiratory health status
Author
Henares, Desirée
Brotons de Los Reyes, Pedro
De Sevilla, Mariona F.
Fernandez-Lopez, Ana
Hernandez-Bou, Susanna
Perez-Argüello, Amaresh
Mira, Alex
Muñoz-Almagro, Carmen
Cabrera-Rubio, Raul
Publication date
2021ISSN
2057-5858
Abstract
Acute respiratory infections (ARIs) constitute one of the leading causes of antibiotic administration, hospitalization and death among children <5 years old. The upper respiratory tract microbiota has been suggested to explain differential susceptibility to ARIs and modulate ARI severity. The aim of the present study was to investigate the relation of nasopharyngeal microbiota and other microbiological parameters with respiratory health and disease, and to assess nasopharyngeal microbiota diagnostic utility for discriminating between different respiratory health statuses. We conducted a prospective case–control study at Hospital Sant Joan de Deu (Barcelona, Spain) from 2014 to 2018. This study included three groups of children <18 years with gradual decrease of ARI severity: cases with invasive pneumococcal disease (IPD) (representative of lower respiratory tract infections and systemic infections), symptomatic controls with mild viral upper respiratory tract infections (URTI), and healthy/asymptomatic controls according to an approximate case–control ratio 1:2. Nasopharyngeal samples were collected from participants for detection, quantification and serotyping of pneumococcal DNA, viral DNA/RNA detection and 16S rRNA gene sequencing. Microbiological parameters were included on case–control classification models. A total of 140 subjects were recruited (IPD=27, URTI=48, healthy/asymptomatic control=65). Children’s nasopharyngeal microbiota composition varied according to respiratory health status and infection severity. The IPD group was characterized by overrepresentation of Streptococcus pneumoniae, higher frequency of invasive pneumococcal serotypes, increased rate of viral infection and underrepresentation of potential protective bacterial species such as Dolosigranulum pigrum and Moraxella lincolnii. Microbiota-based classification models differentiated cases from controls with moderately high accuracy. These results demonstrate the close relationship existing between a child’s nasopharyngeal microbiota and respiratory health, and provide initial evidence of the potential of microbiota-based diagnostics for differential diagnosis of severe ARIs using non-invasive samples.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
61 - Medical sciences
616.2 - Pathology of the respiratory system. Complaints of the respiratory organs
616.9 - Communicable diseases. Infectious and contagious diseases, fevers
Keywords
Infants
Diagnòstic basat en microbiota
Microbiota nasofaríngia
Infeccions respiratòries agudes
Antibiòtics
Microbiota
Malaltia pneumocòcica invasiva
Niños
Diagnóstico basado en microbiota
Microbiota nasofaríngea
Infecciones respiratorias agudas
Antibióticos
Microbiota
Enfermedad neumocócica invasiva
Children
Microbiotic-based diagnosis
Nasopharyngeal microbiota
Acute respiratory infections
Antibiotics
Microbiota
Invasive pneumococcal disease
Pages
17
Publisher
Microbiology Society
Collection
7;10
Is part of
Microbial Genomics
Citation
Henares, Desiree; Brotons de Los Reyes, Pedro; De Sevilla, Mariona F. [et al.]. Differential nasopharyngeal microbiota composition in children according to respiratory health status Open Access. Microbial Genomics, 2021, 7(10), p. 1-17. Disponible en: <https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000661>. Fecha de acceso: 16 dic. 2021. DOI: 10.1099/mgen.0.000661
This item appears in the following Collection(s)
- Ciències de la Salut [725]
Rights
This is an open-access article distributed under the terms of the Creative Commons Attribution License.
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/deed.ast