New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization
Autor/a
Parcerisas, Antoni
Ortega-Gascó, Alba
Hernaiz Llorens, Marc
Odena Caballol, Maria Antonia
Ulloa, Fausto
de Oliveira, Eliandre
Bosch, Miquel
Pujadas, Lluís
Soriano, Eduardo
Fecha de publicación
2021ISSN
1422-0067
Resumen
Neuronal cell adhesion molecule 2 (NCAM2) is a membrane protein with an important role in the morphological development of neurons. In the cortex and the hippocampus, NCAM2 is essential for proper neuronal differentiation, dendritic and axonal outgrowth and synapse formation. However, little is known about NCAM2 functional mechanisms and its interactive partners during brain development. Here we used mass spectrometry to study the molecular interactome of NCAM2 in the second postnatal week of the mouse cerebral cortex. We found that NCAM2 interacts with >100 proteins involved in numerous processes, including neuronal morphogenesis and synaptogenesis. We validated the most relevant interactors, including Neurofilaments (NEFs), Microtubule-associated protein 2 (MAP2), Calcium/calmodulin kinase II alpha (CaMKIIα), Actin and Nogo. An in silico analysis of the cytosolic tail of the NCAM2.1 isoform revealed specific phosphorylation site motifs with a putative affinity for some of these interactors. Our results expand the knowledge of NCAM2 interactome and confirm the key role of NCAM2 in cytoskeleton organization, neuronal morphogenesis and synaptogenesis. These findings are of interest in explaining the phenotypes observed in different pathologies with alterations in the NCAM2 gene.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
54 - Química
57 - Biología
61 - Medicina
Palabras clave
NCAM2
Espectrometria de masses
Citoesquelet
Morfogènesi neuronal
MAPA2
CaMKIIα
Neurofilaments
NCAM2
Espectrometría de masas
Citoesqueleto
Morfogénesis neuronal
MAP2
CaMKIIα
Neurofilamentos
NCAM2
Mass spectrometry
Cytoskeleton
Neuronal morphogenesis
MAP2
CaMKIIα
Neurofilaments
Páginas
20
Publicado por
MDPI
Colección
22;14
Publicado en
International Journal of Molecular Sciences
Citación
Parcerisas, Antoni; Ortega-Gascó, Alba; Hernaiz-Llorens, Marc [et al.]. New partners identified by mass spectrometry assay reveal functions of NCAM2 in neural cytoskeleton organization. International Journal of Molecular Sciences, 2021, 22(14), 7404. Disponible en: <https://www.mdpi.com/1422-0067/22/14/7404>. Fecha de acceso: 9 dic. 2021. DOI: 10.3390/ijms22147404
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