Functional genomics approaches to elucidate vulnerabilities of intrinsic and acquired chemotherapy resistance
Author
Cetin, Ronay
Quandt Herrera, Eva
Kaulich, Manuel
Publication date
2021ISSN
2073-4409
Abstract
Drug resistance is a commonly unavoidable consequence of cancer treatment that results in therapy failure and disease relapse. Intrinsic (pre-existing) or acquired resistance mechanisms can be drug-specific or be applicable to multiple drugs, resulting in multidrug resistance. The presence of drug resistance is, however, tightly coupled to changes in cellular homeostasis, which can lead to resistance-coupled vulnerabilities. Unbiased gene perturbations through RNAi and CRISPR technologies are invaluable tools to establish genotype-to-phenotype relationships at the genome scale. Moreover, their application to cancer cell lines can uncover new vulnerabilities that are associated with resistance mechanisms. Here, we discuss targeted and unbiased RNAi and CRISPR efforts in the discovery of drug resistance mechanisms by focusing on first-in-line chemotherapy and their enforced vulnerabilities, and we present a view forward on which measures should be taken to accelerate their clinical translation.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
61 - Medical sciences
616 - Pathology. Clinical medicine
Keywords
Resistència a la quimioteràpia
Quimioteràpia
Vulnerabilitats del càncer
Càncer
Genòmica funcional
Pantalles RNAi i CRISPR
Resistencia a la quimioterapia
Quimioterapia
Vulnerabilidades al cáncer
Cáncer
Genómica funcional
Pantallas RNAi y CRISPR
Chemotherapy resistance
Chemotherapy
Cancer vulnerabilities
Cancer
Functional genomics
RNAi and CRISPR screens
Pages
27
Publisher
MDPI
Collection
10;2
Is part of
Cells
Citation
Cetin, Ronay; Quandt Herrera, Eva; Kaulich, Manuel. Functional genomics approaches to elucidate vulnerabilities of intrinsic and acquired chemotherapy resistance. Cells, 2021, 10(2), 260. Disponible en: <https://www.mdpi.com/2073-4409/10/2/260>. Fecha de acceso: 13 sep. 2021. DOI: 10.3390/cells10020260
This item appears in the following Collection(s)
- Ciències de la Salut [729]
Rights
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/