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dc.contributor.authorSawadkar, Prasad
dc.contributor.authorMandakhbayar, Nandin
dc.contributor.authorPatel, Kapil D.
dc.contributor.authorOlmas Buitrago, Jennifer
dc.contributor.authorHyun Kim, Tae
dc.contributor.authorRajasekar, Poojitha
dc.contributor.authorLali, Ferdinand
dc.contributor.authorKyriakidis, Christos
dc.contributor.authorRahmani, Benyamin
dc.contributor.authorMohanakrishnan, Jeviya
dc.contributor.authorDua, Rishbha
dc.contributor.authorGreco, Karin
dc.contributor.authorLee, Jung-Hwan
dc.contributor.authorKim, Hae-Won
dc.contributor.authorKnowles, Jonathan C.
dc.contributor.authorGarcía-Gareta, Elena
dc.date.accessioned2021-06-02T17:25:30Z
dc.date.available2021-06-02T17:25:30Z
dc.date.issued2021
dc.identifier.citationSawadkar, Prasad; Mandakhbayar, Nandin; Patel, Kapil D. [et al.]. Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration. Journal of Tissue Engineering, 2021, 12, p. 1-17. Disponible en: <https://journals.sagepub.com/doi/full/10.1177/20417314211019238>. Fecha de acceso: 2 jun. 2021. DOI: 10.1177/20417314211019238ca
dc.identifier.issn2041-7314ca
dc.identifier.urihttp://hdl.handle.net/20.500.12328/2584
dc.description.abstractCurrent gold standard to treat soft tissue injuries caused by trauma and pathological condition are autografts and off the shelf fillers, but they have inherent weaknesses like donor site morbidity, immuno-compatibility and graft failure. To overcome these limitations, tissue-engineered polymers are seeded with stem cells to improve the potential to restore tissue function. However, their interaction with native tissue is poorly understood so far. To study these interactions and improve outcomes, we have fabricated scaffolds from natural polymers (collagen, fibrin and elastin) by custom-designed processes and their material properties such as surface morphology, swelling, wettability and chemical cross-linking ability were characterised. By using 3D scaffolds, we comprehensive assessed survival, proliferation and phenotype of adipose-derived stem cells in vitro. In vivo, scaffolds were seeded with adipose-derived stem cells and implanted in a rodent model, with X-ray microtomography, histology and immunohistochemistry as read-outs. Collagenbased materials showed higher cell adhesion and proliferation in vitro as well as higher adipogenic properties in vivo. In contrast, fibrin demonstrated poor cellular and adipogenesis properties but higher angiogenesis. Elastin formed the most porous scaffold, with cells displaying a non-aggregated morphology in vitro while in vivo elastin was the most degraded scaffold. These findings of how polymers present in the natural polymers mimicking ECM and seeded with stem cells affect adipogenesis in vitro and in vivo can open avenues to design 3D grafts for soft tissue repair.en
dc.format.extent17ca
dc.language.isoengca
dc.publisherSage Journalsca
dc.relation.ispartofJournal of Tissue Engineeringca
dc.relation.ispartofseries12;
dc.rightsCreative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).en
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subject.otherCol·lagenca
dc.subject.otherMaterials biomèdicsca
dc.subject.otherTeixitsca
dc.subject.otherAdipogènesica
dc.subject.otherPolímers naturalsca
dc.subject.otherRegeneració de teixitsca
dc.subject.otherEnginyeria de teixitsca
dc.subject.otherColágenoes
dc.subject.otherMateriales biomédicoses
dc.subject.otherTejidoses
dc.subject.otherAdipogénesises
dc.subject.otherPolímeros naturaleses
dc.subject.otherRegeneración de tejidoses
dc.subject.otherIngeniería de tejidoses
dc.subject.otherCollagenen
dc.subject.otherBiomedical materialsen
dc.subject.otherTissuesen
dc.subject.otherAdipogenesisen
dc.subject.otherNatural polymersen
dc.subject.otherTissue regenerationen
dc.subject.otherTissue engineeringen
dc.titleThree dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regenerationen
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/publishedVersionca
dc.rights.accessLevelinfo:eu-repo/semantics/openAccess
dc.embargo.termscapca
dc.subject.udc61ca
dc.identifier.doihttps://dx.doi.org/10.1177/20417314211019238ca


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Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
Excepte que s'indiqui una altra cosa, la llicència de l'ítem es descriu com https://creativecommons.org/licenses/by-nc/4.0/
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