A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging
Autor/a
Giménez-Gómez, Pablo
Rodríguez-Rodríguez, Rosalía
Ríos, Juan Manuel
Pérez-Montero, Marta
González, Estrella
Gutiérrez-Capitán, Manuel
Plaza, Jose Antonio
Muñoz-Berbel, Xavier
Jiménez-Jorquera, Cecilia
Fecha de publicación
2020ISSN
1473-0189
Resumen
In vitro analysis requires cell proliferation in conditions close to physiological ones. Lab-on-a-chip (LoC) devices simplify, miniaturize and automate traditional protocols, with the advantages of being less expensive and faster due to their shorter diffusion distances. The main limitation of current LoCs is still the control of the culture conditions. Most LoCs employ off-chip equipment to determine cell culture activity, which confers limited monitoring capacity. The few systems integrating transducers on-chip present important functional problems mostly associated with the attachment of biomolecules to the transducer surface (i.e., biofouling) and the impossibility of re-calibrating the sensors during cell culturing. This limitation is addressed in the present LoC containing a network of micro-channels and micro-chambers, which allows (i) cell seeding and cultivation, avoiding biofouling risk, (ii) multiplexed analysis of cell culture, reactivation and recalibration of the (bio)sensors without compromising cell viability, (iii) cell imaging and (iv) reference electrode compartmentalization to guarantee stability. The activity of the culture is monitored with four independent electrochemical micro-electrodes for glucose, hydrogen peroxide, conductivity and oxidation reduction potential. Electrochemical analysis is complemented with high-resolution confocal microscopy analysis. This paper demonstrates the suitability of the current configuration for cell culture monitoring and future applications in drug screening or organ-on-a-chip development.
Tipo de documento
Artículo
Versión del documento
Versión aceptada
Lengua
English
Materias (CDU)
61 - Medicina
Palabras clave
Cultius cel·lulars
Anàlisi electroquímica
Estrès oxidatiu
Cell culture
Electrochemical analysis
Oxidative stress
Cultivo celular
Análisis electroquímico
Estrés oxidativo
Páginas
11
Publicado por
Royal Society of Chemistry
Colección
20;
Publicado en
Lab on a Chip
Citación
Giménez-Gómez, Pablo; Rodríguez-Rodríguez, Rosalía; Ríos, Juan Manuel [et al.]. A self-calibrating and multiplexed electrochemical lab-on-a-chip for cell culture analysis and high-resolution imaging. Lab on a Chip, 2020, vol. 20, p. 823-833. Disponible en: <https://pubs.rsc.org/en/content/articlelanding/2020/LC/C9LC01051C#!divAbstract>. Fecha de acceso: 1 mar. 2020. DOI: 10.1039/C9LC01051C
Número del acuerdo de la subvención
info:eu-repo/grantAgreement//ES/1PE/TEC2014-54449-C3-1-R
info:eu-repo/grantAgreement//ES/2PE/RTI2018-100773-B-C31
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