A nucleotide-based drug protects against glutamate- and MPP+ -induced neurotoxicity
Author
Gella Concustell, Alejandro
Martiáñez Canales, Tània
Lamarca Dams, Aloa
Gutierrez, Cristina
Durany, Nuria
Casals i Farré, Núria
Publication date
2011ISSN
2158-2912
Abstract
Nucleo CMP Forte® is a nucleotide-based drug consisting of cytidinemonophosphate, uridinemonophosphate, uridin-ediphosphate and uridinetriphosphate. It has been prescribed for peripheral nervous system disorders, such as lum-bosciatalgia, diabetic or alcoholic polyneuropathy, or trigeminal neuralgia. Its effects on brain pathologies has re-ceived little attention. We examined its neuroprotective effects on cell toxicity induced by glutamate excitotoxicity or by 1-methyl-4-phenyl-pyridinium (MPP+), an in vitro cell model of Parkinson’s disease. We used the human dopaminergic cell line SH-SY5Y and a primary culture of rat cortical cells pre-treated with the drug for 24 hours and then exposed to MPP+ or glutamate at a range of concentrations. Cell viability was measured at different times. Nucleo CMP Forte® pre-treatment significantly increased the rate of cell division in SH-SY5Y cells, as well as the synthesis of triglycerides and phospholipids. More interestingly, drug pre-treatment significantly reduced MPP+- and glutamate-induced cell death in SH-SY5Y cells and in rat cortical cells. These results indicate that the nucleotides included in Nucleo CMP Forte® are promising therapeutic molecules for the prevention of neuronal death in brain caused by focal ischemia, Parkinson’s disease or other neurodegenerative pathologies.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
61 - Medical sciences
Keywords
Nucleòtids
Dany cerebral
Aminoàcids
Parkinson, Malaltia de
Nucleótidos
Daño cerebral
Aminoácidos
Parkinson, Enfermedad de
Nucleotides
Brain damage
Amino acids
Parkinson's disease
Pages
7
Publisher
Scientific Research
Collection
2;
Is part of
Neuroscience & Medicine
Citation
Gella, Alejandro; Martiañez, Tania; Lamarca, Aloa [et al.]. A nucleotide-based drug protects against glutamate- and MPP+ -induced neurotoxicity. Neuroscience & Medicine, 2011, vol. 2, p. 154-160. Disponible en: <https://www.scirp.org/journal/paperinformation.aspx?paperid=5538>. Fecha de acceso: 22 feb. 2020. DOI: 10.4236/nm.2011.22022
Grant agreement number
info:eu-repo/grantAgreement/ES/1PN/BIO2002-00128
info:eu-repo/grantAgreement/ES/1PN/BIO2005-01591
This item appears in the following Collection(s)
- Ciències de la Salut [740]
Rights
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Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/