Comprehensive and quantitative analysis of G1 cyclins. A tool for studying the cell cycle
Autor/a
Ballega, Elisabet
Carballar Ruiz, María de los Reyes
Samper Martín, Bàrbara
Ricco Pacheco, Natalia
Ribeiro, Mariana P. C.
Jiménez, Javier
Clotet Erra, Josep
Fecha de publicación
2019-06-25ISSN
1932-6203
Resumen
In eukaryotes, the cell cycle is driven by the actions of several cyclin dependent kinases (CDKs) and an array of regulatory proteins called cyclins, due to the cyclical expression patterns of the latter. In yeast, the accepted pattern of cyclin waves is based on qualitative studies performed by different laboratories using different strain backgrounds, different growing conditions and media, and different kinds of genetic manipulation. Additionally, only the subset of cyclins regulating Cdc28 was included, while the Pho85 cyclins were excluded. We describe a comprehensive, quantitative and accurate blueprint of G1 cyclins in the yeast Saccharomyces cerevisiae that, in addition to validating previous conclusions, yields new findings and establishes an accurate G1 cyclin blueprint. For the purposes of this research, we produced a collection of strains with all G1 cyclins identically tagged using the same and most respectful procedure possible. We report the contribution of each G1 cyclin for a broad array of growing and stress conditions, describe an unknown role for Pcl2 in heat-stress conditions and demonstrate the importance of maintaining the 3’UTR sequence of cyclins untouched during the tagging process.
Tipo de documento
Artículo
Versión del documento
Versión aceptada
Lengua
English
Materias (CDU)
576 - Biología celular y subcelular. Citología
61 - Medicina
Palabras clave
Osmosi
Experiments científics
Expressió gènica
Cèl·lules
Ósmosis
Experimentos científicos
Expresión génica
Células
Ósmosis
Cells
Experiments
Gene expression
Páginas
24
Publicado por
Public Library of Science
Colección
14; 6
Publicado en
PLoS One
Citación
Ballega, Elisabet; Carballar, Reyes; Samper, Bàrbara [et al.]. Comprehensive and quantitative analysis of G1 cyclins. A tool for studying the cell cycle. PLoS One, 2018, vol. 14, núm. 6, e0218531. Disponible en: <https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0218531>. Fecha de acceso: 2 feb. 2020. DOI: 10.1371/journal.pone.0218531
Número del acuerdo de la subvención
info:eu-repo/grantAgreement/ES/1PE/BFU2013-44189-P
info:eu-repo/grantAgreement/ES/2PE/PGC2018-096597-B-I00
info:eu-repo/grantAgreement/ES/2PE/PGC2018-096597-B-I00
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Derechos
© 2019 Ballega et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Excepto si se señala otra cosa, la licencia del ítem se describe como https://creativecommons.org/licenses/by/4.0/