Centromeric signaling proteins boost G1 cyclin degradation and modulate cell size in budding yeast
Author
Martínez-Láinez, Joan M.
Moreno, David F.
Parisi, Eva
Clotet Erra, Josep
Aldea Malo, Martí
Publication date
2018-08-06ISSN
1544-9173
Abstract
Cell size scales with ploidy in a great range of eukaryotes, but the underlying mechanisms
remain unknown. Using various orthogonal single-cell approaches, we show that cell size
increases linearly with centromere (CEN) copy number in budding yeast. This effect is due
to a G1 delay mediated by increased degradation of Cln3, the most upstream G1 cyclin acting
at Start, and specific centromeric signaling proteins, namely Mad3 and Bub3. Mad3
binds both Cln3 and Cdc4, the adaptor component of the Skp1/Cul1/F-box (SCF) complex
that targets Cln3 for degradation, these interactions being essential for the CEN-dosage
dependent effects on cell size. Our results reveal a pathway that modulates cell size as a
function of CEN number, and we speculate that, in cooperation with other CEN-independent
mechanisms, it could assist the cell to attain efficient mass/ploidy ratios
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
61 - Medical sciences
Keywords
Genomes
Genomas
Cells
Cèl·lules
Células
Pages
13
Publisher
Public Library of Science
Collection
16; 8
Is part of
PLoS Biology
Citation
Martínez-Láinez, Joan M.; Moreno, David F.; Parisi, Eva; Clotet, Josep; Aldea Malo, Martí. «Centromeric signaling proteins boost G1 cyclin degradation and modulate cell size in budding yeast». PLoS Biology, 2018, vol. 16, núm. 8, art. e2005388. Disponible en: <https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.2005388>. Fecha de acceso: 24 oct. 2019. DOI: 10.1371/journal.pbio.2005388
This item appears in the following Collection(s)
- Ciències de la Salut [740]
Rights
http://creativecommons.org/licenses/by-nc-nd/4.0/
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/