X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk
Author
Fadó Andrés, Rut
Moubarak, Rana S.
Miñano Molina, Alfredo Jesús
Barneda-Zahonero, Bruna
Valero, Jorge
Saura, Carlos A.
Moran, Julio
Comella, Joan X.
Rodríguez-Álvarez, José
Publication date
2013-08-09ISSN
2045-2322
Abstract
X-linked Inhibitor of apoptosis protein (XIAP) has been classically identified as a cell death regulator. Here,
we demonstrate a novel function of XIAP as a regulator of neurite outgrowth in neuronal cells. In PC12 cells,
XIAP overexpression prevents NGF-induced neuronal differentiation, whereas NGF treatment induces a
reduction of endogenous XIAP levels concomitant with the induction of neuronal differentiation.
Accordingly, downregulation of endogenous XIAP protein levels strongly increases neurite outgrowth in
PC12 cells as well as axonal and dendritic length in primary cortical neurons. The effects of XIAP are
mediated by the mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinases (ERKs)
pathway since blocking this pathway completely prevents the neuritogenesis mediated by XIAP
downregulation. In addition, we found that XIAP binds to cRaf and Trk receptors. Our results demonstrate
that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal
differentiation in developing neurons.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
61 - Medical sciences
Keywords
Neurociencias
Neurociències
Neurosciences
Inhibició
Inhibition
Apoptosi
Apoptosis
Pages
11
Publisher
Nature Research
Collection
3;
Is part of
Scientific Reports
Citation
Fadó Andrés, Rut; Moubarak, Rana S.; Miñano-Molina, Alfredo J.; Barneda-Zahonero, Bruna; Valero, Jorge; Saura, Carlos A.; Moran, Julio; Comella, Joan X.; Rodríguez-Álvarez, José. «X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk». Scientific Reports, 2013, vol. 3, art. 2397. Disponible en: <https://www.nature.com/articles/srep02397>. Fecha de acceso: 16 oct. 2019
This item appears in the following Collection(s)
- Ciències de la Salut [745]
Rights
http://creativecommons.org/licenses/by-nc-nd/4.0/
Except where otherwise noted, this item's license is described as http://creativecommons.org/licenses/by-nc-nd/4.0/