Dental pulp of the third molar: a new source of pluripotent-like stem cells
Autor/a
Atari, Maher
Gil Recio, Carlos
Fabregat Navarro, Marc
García Fernández, Daniel
Barajas Vélez, Miguel
Carrasco Garcia, Miguel Ángel
Jung, Han-Sung
Hernández Alfaro, Federico
Casals i Farré, Núria
Prósper, Felipe
Ferrés-Padró, Eduard
Data de publicació
2012-09-05ISSN
0021-9533
Resum
Dental pulp is particularly interesting in regenerative medicine because of the accessibility and differentiation potential of the tissue.
Dental pulp has an early developmental origin with multi-lineage differentiation potential as a result of its development during
childhood and adolescence. However, no study has previously identified the presence of stem cell populations with embryonic-like
phenotypes in human dental pulp from the third molar. In the present work, we describe a new population of dental pulp pluripotent-like
stem cells (DPPSCs) that were isolated by culture in medium containing LIF, EGF and PDGF. These cells are SSEA4+, OCT3/4+,
NANOG+, SOX2+, LIN28+, CD13+, CD105+, CD342, CD452, CD90+, CD29+, CD73+, STRO1+ and CD1462, and they show genetic
stability in vitro based on genomic analysis with a newly described CGH technique. Interestingly, DPPSCs were able to form both
embryoid-body-like structures (EBs) in vitro and teratoma-like structures that contained tissues derived from all three embryonic germ
layers when injected in nude mice. We examined the capacity of DPPSCs to differentiate in vitro into tissues that have similar
characteristics to mesoderm, endoderm and ectoderm layers in both 2D and 3D cultures. We performed a comparative RT-PCR analysis
of GATA4, GATA6, MIXL1, NANOG, OCT3/4, SOX1 and SOX2 to determine the degree of similarity between DPPSCs, EBs and human
induced pluripotent stem cells (hIPSCs). Our analysis revealed that DPPSCs, hIPSC and EBs have the same gene expression profile.
Because DPPSCs can be derived from healthy human molars from patients of different sexes and ages, they represent an easily
accessible source of stem cells, which opens a range of new possibilities for regenerative medicine.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
Anglès
Matèries (CDU)
616.3 - Patologia de l'aparell digestiu. Odontologia
Paraules clau
Polpa dental
Dental pulp
Pulpa dental
Cèl·lules mare
Stem cells
Células madre
DPPSC
CGH technique
Pàgines
14-24
Publicat per
Company of Biologists
Col·lecció
125;14
Publicat a
Journal of Cell Science
Citació
Atari, Maher; Gil Recio, Carlos; Fabregat, Marc; García Fernández, Daniel; Barajas, Miguel; Carrasco García, Miguel Ángel; Jung, Han-Sung; Hernández Alfaro, Federico; Casals Farré, Núria; Prósper, Felipe; Ferrés Padró, Eduard; Giner Tarrida, Lluís. «Dental pulp of the third molar: a new source of pluripotent-like stem cells». Journal of Cell Science, 2012, vol. 125, núm. 14, p. 3343–3356. Disponible en: <https://jcs.biologists.org/content/125/14/3343>. Fecha de acceso: 19 sept. 2019. http://dx.doi.org/10.1242/jcs.096537
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- Odontologia [228]
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