| dc.contributor.author | Sanchez, Mayka | |
| dc.contributor.author | Galy, Bruno | |
| dc.contributor.author | Hentze, Matthias W. | |
| dc.contributor.author | Muckenthaler, Martina U. | |
| dc.date.accessioned | 2024-01-31T14:15:13Z | |
| dc.date.available | 2024-01-31T14:15:13Z | |
| dc.date.issued | 2007 | |
| dc.identifier.citation | Sanchez, Mayka; Galy, Bruno; Hentze, Matthias W. [et al.]. Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays. Nature Protocols, 2007, 2, p. 2033–2042. Disponible en: <https://www.nature.com/articles/nprot.2007.293>. Fecha de acceso: 31 ene. 2024. DOI: 10.1038/nprot.2007.293 | ca |
| dc.identifier.uri | http://hdl.handle.net/20.500.12328/3997 | |
| dc.description.abstract | RNA-binding proteins (RBPs) frequently regulate the post-transcriptional fate of target mRNAs. To identify novel target mRNAs of RBPs, we incubate total RNA with recombinant RBP and immunoselect the messenger-ribonucleoproteins using a specific anti-RBP antibody. The mRNA composition of the supernatant and/or immunoprecipitated fraction is analyzed using dual-color microarrays in comparison with control reaction. From start to finish, the protocol takes approximately 6 d. | ca |
| dc.format.extent | 9 | ca |
| dc.language.iso | eng | ca |
| dc.publisher | Springer Nature | ca |
| dc.relation.ispartof | Nature Protocols | ca |
| dc.relation.ispartofseries | 2 | |
| dc.subject.other | Medicina | ca |
| dc.subject.other | Medicine | ca |
| dc.title | Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays | ca |
| dc.type | info:eu-repo/semantics/article | ca |
| dc.description.version | info:eu-repo/semantics/publishedVersion | ca |
| dc.rights.accessLevel | info:eu-repo/semantics/openAccess | |
| dc.embargo.terms | cap | ca |
| dc.subject.udc | 61 | ca |
| dc.identifier.doi | https://dx.doi.org/10.1038/nprot.2007.293 | ca |
