Human whole blood interactions with craniomaxillofacial reconstruction materials: exploring In vitro the role of blood cascades and leukocytes in early healing events
Autor/a
Anand, Vivek
Gallinetti, Sara
Aparicio, Conrado
Fecha de publicación
2023ISSN
2079-4983
Resumen
The present study investigated early interactions between three alloplastic materials (calcium phosphate (CaP), titanium alloy (Ti), and polyetheretherketone (PEEK) with human whole blood using an established in vitro slide chamber model. After 60 min of contact with blood, coagulation (thrombin–antithrombin complexes, TAT) was initiated on all test materials (Ti > PEEK > CaP), with a significant increase only for Ti. All materials showed increased contact activation, with the KK–AT complex significantly increasing for CaP (p < 0.001), Ti (p < 0.01), and PEEK (p < 0.01) while only CaP demonstrated a notable rise in KK-C1INH production (p < 0.01). The complement system had significant activation across all materials, with CaP (p < 0.0001, p < 0.0001) generating the most pronounced levels of C3a and sC5b-9, followed by Ti (p < 0.001, p < 0.001) and lastly, PEEK (p < 0.001, p < 0.01). This activation correlated with leukocyte stimulation, particularly myeloperoxidase release. Consequently, the complement system may assume a more significant role in the early stages post implantation in response to CaP materials than previously recognized. Activation of the complement system and the inevitable activation of leukocytes might provide a more favorable environment for tissue remodeling and repair than has been traditionally acknowledged. While these findings are limited to the early blood response, complement and leukocyte activation suggest improved healing outcomes, which may impact long-term clinical outcomes.
Tipo de documento
Artículo
Versión del documento
Versión publicada
Lengua
Inglés
Materias (CDU)
616.3 - Patología del aparato digestivo. Odontología
Palabras clave
Biomaterials
Sang sencera humana
Coagulació
Complement
Fosfat de calci
Biomateriales
Sangre entera humana
Coagulación
Complementar
Fosfato de calcio
Biomaterials
Human whole blood
Coagulation
Complement
Calcium phosphate
Páginas
17
Publicado por
MDPI
Colección
14;7
Publicado en
Journal of Functional Biomaterials
Citación
Lopes, Viviana; Birgersson, Ulrik; Anand, Vivek [et al.]. Human whole blood interactions with craniomaxillofacial reconstruction materials: exploring In vitro the role of blood cascades and leukocytes in early healing events. Journal of Functional Biomaterials, 2023, 14(7), 361. Disponible en: <https://www.mdpi.com/2079-4983/14/7/361>. Fecha de acceso: 14 nov. 2023. DOI: 10.3390/jfb14070361.
Este ítem aparece en la(s) siguiente(s) colección(ones)
- Odontologia [228]
Derechos
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Excepto si se señala otra cosa, la licencia del ítem se describe como https://creativecommons.org/licenses/by/4.0/