Titanium surface characteristics induce the specific reprogramming of toll-like receptor signaling in macrophages
Author
González-Sánchez, Zaira
Areal-Quecuty, Victoria
Jimenez-Guerra, Alvaro
Pozo Perez, David
Publication date
2022ISSN
1422-0067
Abstract
Most of the research on titanium-based dental implants (Ti-discs) is focused on how they are able to stimulate the formation of new tissue and/or cytotoxic studies, with very scarce data on their effects on functional responses by immunocompetent cells. In particular, the link between the rewiring of innate immune responses and surface biomaterials properties is poorly understood. To address this, we characterize the functional response of macrophage cultures to four different dental titanium surfaces (MA: mechanical abrasion; SB + AE: sandblasting plus etching; SB: sandblasting; AE: acid etching). We use different Toll-like receptor (TLR) ligands towards cell surface receptors (bacterial lipopolysaccharide LPS for TLR4; imiquimod for TLR7; synthetic bacterial triacylated lipoprotein for TLR2/TLR1) and endosomal membrane receptor (poly I:C for TLR3) to simulate bacterial (cell wall bacterial components) or viral infections (dsRNA and ssRNA). The extracellular and total LDH levels indicate that exposure to the different Ti-surfaces is not cytotoxic for macrophages under resting or TLR-stimulated conditions, although there is a tendency towards an impairment in macrophage proliferation, viability or adhesion under TLR4, TLR3 and TLR2/1 stimulations in SB discs cultures. The secreted IL-6 and IL-10 levels are not modified upon resting macrophage exposure to the Ti-surfaces studied as well as steady state levels of iNos or ArgI mRNA. However, macrophage exposure to MA Ti-surface do display an enhanced immune response to TLR4, TLR7 or TLR2/1 compared to other Ti-surfaces in terms of soluble immune mediators secreted and M1/M2 gene expression profiling. This change of characteristics in cellular phenotype might be related to changes in cellular morphology. Remarkably, the gene expression of Tlr3 is the only TLR that is differentially affected by distinct Ti-surface exposure. These results highlight the relevance of patterned substrates in dental implants to achieve a smart manipulation of the immune responses in the context of personalized medicine, cell-based therapies, preferential lineage commitment of precursor cells or control of tissue architecture in oral biology.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Discs de titani
Implants dentals
Superfícies
Regulació immune
Cèl·lules macròfags
Receptors de tipus toll
Discos de titanio
Implantes dentales
Superficies
Regulación inmune
Células macrófagas
Receptores tipo Toll
Titanium discs
Dental implants
Surfaces
Immune regulation
Macrophage cells
Toll-like receptors
Pages
15
Publisher
MDPI
Collection
23;8
Is part of
International Journal of Molecular Sciences
Citation
González-Sánchez, Zaira; Areal-Quecuty, Victoria; Jimenez-Guerra, Alvaro [et al.]. Titanium surface characteristics induce the specific reprogramming of toll-like receptor signaling in macrophages. International Journal of Molecular Sciences, 2022, 23(8), 4285. Disponible en: <https://www.mdpi.com/1422-0067/23/8/4285>. Fecha de acceso: 24 feb. 2023. DOI: 10.3390/ijms23084285
Grant agreement number
info:eu-repo/grantAgreement/ES/2PE/RTI2018-098075-B-C22
info:eu-repo/grantAgreement/ES/2PE/RTI2018-098432-BI00
Link to the related item
This item appears in the following Collection(s)
- Odontologia [228]
Rights
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/