Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation

Cabré, Gisela; Garrido-Charles, Aida; Moreno, Miquel; Bosch, Miquel; Porta-de-la-Riva, Montserrat; Krieg, Michael; Gascón-Moya, Marta; Camarero, Núria; Gelabert, Ricard; Lluch, José M.; Busqué, Félix; Hernando, Jordi; Gorostiza, Pau; Alibés, Ramon

dc.contributor.author	Cabré, Gisela
dc.contributor.author	Garrido-Charles, Aida
dc.contributor.author	Moreno, Miquel
dc.contributor.author	Bosch, Miquel
dc.contributor.author	Porta-de-la-Riva, Montserrat
dc.contributor.author	Krieg, Michael
dc.contributor.author	Gascón-Moya, Marta
dc.contributor.author	Camarero, Núria
dc.contributor.author	Gelabert, Ricard
dc.contributor.author	Lluch, José M.
dc.contributor.author	Busqué, Félix
dc.contributor.author	Hernando, Jordi
dc.contributor.author	Gorostiza, Pau
dc.contributor.author	Alibés, Ramon
dc.date.accessioned	2023-02-02T15:29:20Z
dc.date.available	2023-02-02T15:29:20Z
dc.date.issued	2019
dc.identifier.citation	Cabré, Gisela; Garrido-Charles, Aida; Moreno, Miquel [et al]. Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation. Nature Communications, 2019, 10, 907. Disponible en: <https://www.nature.com/articles/s41467-019-08796-9>. Fecha de acceso: 2 feb. 2023. DOI: 10.1038/s41467-019-08796-9	ca
dc.identifier.issn	2041-1723	ca
dc.identifier.uri	http://hdl.handle.net/20.500.12328/3546
dc.description.abstract	Manipulation of neuronal activity using two-photon excitation of azobenzene photoswitches with near-infrared light has been recently demonstrated, but their practical use in neuronal tissue to photostimulate individual neurons with three-dimensional precision has been hampered by firstly, the low efficacy and reliability of NIR-induced azobenzene photoisomerization compared to one-photon excitation, and secondly, the short cis state lifetime of the two-photon responsive azo switches. Here we report the rational design based on theoretical calculations and the synthesis of azobenzene photoswitches endowed with both high two-photon absorption cross section and slow thermal back-isomerization. These compounds provide optimized and sustained two-photon neuronal stimulation both in light-scattering brain tissue and in Caenorhabditis elegans nematodes, displaying photoresponse intensities that are comparable to those achieved under one-photon excitation. This finding opens the way to use both genetically targeted and pharmacologically selective azobenzene photoswitches to dissect intact neuronal circuits in three dimensions.	en
dc.format.extent	12	ca
dc.language.iso	eng	ca
dc.publisher	Springer Nature	ca
dc.relation.ispartof	Nature Communications	ca
dc.relation.ispartofseries	10
dc.relation.uri	https://www.nature.com/articles/s41467-019-08796-9	ca
dc.rights	This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.	en
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject.other	Canals de calci	ca
dc.subject.other	Eines químiques	ca
dc.subject.other	Fotobiologia	ca
dc.subject.other	Canales de calcio	es
dc.subject.other	Herramientas químicas	es
dc.subject.other	Fotobiología	es
dc.subject.other	Calcium channels	en
dc.subject.other	Chemical tools	en
dc.subject.other	Photobiology	en
dc.title	Rationally designed azobenzene photoswitches for efficient two-photon neuronal excitation	en
dc.type	info:eu-repo/semantics/article	ca
dc.description.version	info:eu-repo/semantics/publishedVersion	ca
dc.rights.accessLevel	info:eu-repo/semantics/openAccess
dc.embargo.terms	cap	ca
dc.subject.udc	6	ca
dc.identifier.doi	https://dx.doi.org/10.1038/s41467-019-08796-9	ca

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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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