Immobilization of BMP-2 and VEGF within multilayered polydopamine-coated scaffolds and the resulting osteogenic and angiogenic synergy of co-cultured human mesenchymal stem cells and human endothelial progenitor cells
Author
Godoy-Gallardo, Maria
Portolés-Gil, Núria
López-Periago, Ana M.
Domingo, Concepción
Hosta-Rigau, Leticia
Publication date
2020ISSN
1422-0067
Abstract
We have previously reported the fabrication of a polycaprolactone and hydroxyapatite composite scaffold incorporating growth factors to be used for bone regeneration. Two growth factors were incorporated employing a multilayered coating based on polydopamine (PDA). In particular, Bone morphogenetic protein-2 (BMP-2) was bound onto the inner PDA layer while vascular endothelial growth factor (VEGF) was immobilized onto the outer one. Herein, the in vitro release of both growth factors is evaluated. A fastest VEGF delivery followed by a slow and more sustained release of BMP-2 was demonstrated, thus fitting the needs for bone tissue engineering applications. Due to the relevance of the crosstalk between bone-promoting and vessel-forming cells during bone healing, the functionalized scaffolds are further assessed on a co-culture setup of human mesenchymal stem cells and human endothelial progenitor cells. Osteogenic and angiogenic gene expression analysis indicates a synergistic effect between the growth factor-loaded scaffolds and the co-culture conditions. Taken together, these results indicate that the developed scaffolds hold great potential as an efficient platform for bone-tissue applications.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
61 - Medical sciences
616.7 - Pathology of the organs of locomotion. Skeletal and locomotor systems
Keywords
Angiogènesi
Enginyeria del teixit ossi
Coentrega
Factors de creixement
Multicapa
Osteogènesi
Polidopamina
Bastides
Angiogénesis
Ingeniería de tejidos óseos
Co-entrega
Factores de crecimiento
Multicapas
Osteogénesis
Polidopamina
Andamios
Angiogenesis
Bone tissue engineering
Co-delivery
Growth factors
Multilayers
Osteogenesis
Polydopamine
Scaffolds
Pages
25
Publisher
MDPI
Collection
21; 17
Is part of
International Journal of Molecular Sciences
Citation
Godoy-Gallardo, Maria; Portolés-Gil, Núria; López-Periago, Ana M. [et al.]. Immobilization of BMP-2 and VEGF within multilayered polydopamine-coated scaffolds and the resulting osteogenic and angiogenic synergy of co-cultured human mesenchymal stem cells and human endothelial progenitor cells. International Journal of Molecular Sciences, 2020, 21(17), 6418. Disponible en: <https://www.mdpi.com/1422-0067/21/17/6418>. Fecha de acceso: 17 ene. 2022. DOI: 10.3390/ijms21176418
This item appears in the following Collection(s)
- Ciències de la Salut [740]
Rights
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/