Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis
Autor/a
Castellanos, Maria Isabel
Mas-Moruno, Carlos
DIAZ-RICART, MARIBEL
Escolar Albaladejo, Ginés
Ginebra, Maria-Pau
Gil Mur, Francisco Javier
PEGUEROLES, MARTA
Data de publicació
2017ISSN
1549-3296
Resum
Immobilization of bioactive peptide sequences on CoCr surfaces is an effective route to improve endothelialization, which is of great interest for cardiovascular stents. In this work, we explored the effect of physical and covalent immoblization of RGDS, YIGSR and their equimolar combination peptides on endothelial cells (EC) and smooth muscle cell (SMC) adhesion and on thrombogenicity. We extensively investigated using RT-qPCR, the expression by ECs cultured on functionalised CoCr surfaces of different genes. Genes relevant for adhesion (ICAM-1 and VCAM-1), vascularization (VEGFA, VEGFR-1 and VEGFR-2) and anti-thrombogenicity (tPA and eNOS) were over-expressed in the ECs grown to covalently functionalized CoCr surfaces compared to physisorbed and control surfaces. Pro-thrombogenic genes expression (PAI-1 and vWF) decreased over time. Cell co-cultures of ECs/SMCs found that functionalization increased the amount of adhered ECs onto modified surfaces compared to plain CoCr, independently of the used peptide and the strategy of immobilization. SMCs adhered less compared to ECs in all surfaces. All studied peptides showed a lower platelet cell adhesion compared to TCPS. Covalent functionalization of CoCr surfaces with an equimolar combination of RGDS and YIGSR represented prevailing strategy to enhance the early stages of ECs adhesion and proliferation, while preventing SMCs and platelet adhesion.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
Anglès
Matèries (CDU)
616.3 - Patologia de l'aparell digestiu. Odontologia
Paraules clau
Pèptids adhesius cel·lulars
Aliatge de CoCr
Endotelització
Trombogènesi
Restenosi
Péptidos adhesivos celulares
Aleación de CoCr
Endotelización
Trombogénesis
Restenosis
Cell adhesive peptides
CoCr alloy
Endothelialization
Thrombogenesis
Restenosis
Pàgines
11
Publicat per
John Wiley & Sons
Col·lecció
105;4
Publicat a
Journal of biomedical materials research. Part A
Citació
Castellanos, Maria Isabel; Guillem Martí, Jordi; Mas Moruno, Carlos [et al.]. Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis. Journal of biomedical materials research. Part A, 2017, 105(4), p. 973-983. Disponible en: <https://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35988>. Fecha de acceso: 16 nov. 2021. DOI: 10.1002/jbm.a.35988
Aquest element apareix en la col·lecció o col·leccions següent(s)
- Odontologia [228]
Drets
© 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 973–983, 2017.