Tuning mesenchymal stem cell response onto titanium-niobium-hafnium alloy by recombinant fibronectin fragments
Author
Herranz Díez, Carolina
Mas-Moruno, Carlos
Neubauer, Stefanie
Kessler, Horst
Gil Mur, Francisco Javier
PEGUEROLES, MARTA
Manero, José María
Guillem-Marti, Jordi
Publication date
2016ISSN
1944-8244
Abstract
Since metallic biomaterials used for bone replacement possess low bioactivity, the use of cell adhesive moieties is a common strategy to improve cellular response onto these surfaces. In recent years, the use of recombinant proteins has emerged as an alternative to native proteins and short peptides owing to the fact that they retain the biological potency of native proteins, while improving their stability. In the present study, we investigated the biological effect of two different recombinant fragments of fibronectin, spanning the 8–10th and 12–14th type III repeats, covalently attached to a new TiNbHf alloy using APTES silanization. The fragments were studied separately and mixed at different concentrations and compared to a linear RGD, a cyclic RGD and the full-length fibronectin protein. Cell culture studies using rat mesenchymal stem cells demonstrated that low to medium concentrations (30% and 50%) of type III 8–10th fragment mixed with type III 12–14th fragment stimulated cell spreading and proliferation compared to RGD peptides and the fragments separately. On the other hand, type III 12–14th fragment alone or mixed at low volume percentages ≤50% with type III 8–10th fragment increased alkaline phosphatase levels compared to the other molecules. These results are significant for the understanding of the role of fibronectin recombinant fragments in cell responses and thus to design bioactive coatings for biomedical applications.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Pèptids
Adhesius de cèl·lules de proteïna recombinant
Fibronectina
Cèl·lules mare
Mesenquimals
Aliatge de titani
Péptidos
Pegatinas de células de proteína recombinante
Fibronectina
Células madre
Mesenquimales
Aleación de titanio
Peptides
Recombinant protein cell adhesives
Fibronectin
Stem cells
Mesenchymals
Titanium alloy
Pages
41
Publisher
ACS Publications
Collection
8;4
Is part of
ACS Applied Materials and Interfaces
Citation
Herranz-Diez, Carolina; Mas Moruno, Carlos; Neubauer, S. [et al.]. Tuning mesenchymal stem cell response onto titanium-niobium-hafnium alloy by recombinant fibronectin fragments. ACS Applied Materials and Interfaces, 2016, 8(4), p. 2517-2525. Disponible en: <https://pubs.acs.org/doi/10.1021/acsami.5b09576>. Fecha de acceso: 15 nov. 2021. DOI: 10.1021/acsami.5b09576
Grant agreement number
info:eu-repo/grantAgreement/MINECO/MAT2012-30706
info:eu-repo/grantAgreement/EC/FP7/321985
This item appears in the following Collection(s)
- Odontologia [228]
Rights
© 2021 American Chemical Society