Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization
Author
Godoy-Gallardo, Maria
Mas-Moruno, Carlos
Yu, Kai
Manero, José María
Gil Mur, Francisco Javier
Kizhakkedathu, Jayachandran
Rodríguez Rius, Daniel
Publication date
2015ISSN
1525-7797
Abstract
Dental implant failure can be associated with infections that develop into peri-implantitis. In order to reduce biofilm formation, several strategies focusing on the use of antimicrobial peptides (AMPs) have been studied. To covalently immobilize these molecules onto metallic substrates, several techniques have been developed, including silanization and polymer brush prepared by surface-initiated atom transfer radical polymerization (ATRP), with varied peptide binding yield and antibacterial performance. The aim of the present study was to compare the efficiency of these methods to immobilize the lactoferrin-derived hLf1–11 antibacterial peptide onto titanium, and evaluate their antibacterial activity in vitro. Smooth titanium samples were coated with hLf1–11 peptide under three different conditions: silanization with 3-aminopropyltriethoxysilane (APTES), and polymer brush based coatings with two different silanes. Peptide presence was determined by X-ray photoelectron spectroscopy, and the mechanical stability of the coatings was studied under ultrasonication. The LDH assays confirmed that HFFs viability and proliferation were no affected by the treatments. The in vitro antibacterial properties of the modified surfaces were tested with two oral strains (Streptococcus sanguinis and Lactobacillus salivarius) showing an outstanding reduction. A higher decrease in bacterial attachment was noticed when samples were modified by ATRP methods compared to silanization. This effect is likely due to the capacity to immobilize more peptide on the surfaces using polymer brushes and the nonfouling nature of polymer PDMA segment.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Materials de recobriment
Biofilms
Pèptids i proteïnes
Titani
Polimerització radical
Implants dentals
Materiales de revestimiento
Biofilms
Péptidos y proteínas
Titanio
Polimerización radical
Implantes dentales
Coating materials
Biofilms
Peptides and proteins
Titanium
Radical polymerization
Dental implants
Pages
13
Publisher
ACS Publications
Collection
2;
Is part of
Biomacromolecules
Citation
Godoy Gallardo, Maria; Mas Moruno, Carlos; Yu, Kai [et al.]. Antibacterial properties of hLf1-11 peptide onto titanium surfaces: a comparison study between silanization and surface initiated polymerization. Biomacromolecules, 2015, 16(2), p. 483-496. Disponible en: <https://pubs.acs.org/doi/10.1021/bm501528x#>. Fecha de acceso: 15 nov. 2021. DOI: 10.1021/bm501528x
Grant agreement number
info:eu-repo/grantAgreement/MICINN/MAT2009-12547
info:eu-repo/grantAgreement/MINECO/MAT2012-30706
info:eu-repo/grantAgreement/EC/FP7/321985
This item appears in the following Collection(s)
- Odontologia [228]
Rights
© 2014 American Chemical Society