Silver deposition on titanium surface by electrochemical anodizing process reduces bacterial adhesion of Streptococcus sanguinis and Lactobacillus salivarius
Author
Godoy-Gallardo, Maria
Rodríguez Hernández, Ana Guadalupe
Delgado, Luis M.
Manero, José María
Gil Mur, Francisco Javier
Rodríguez Rius, Daniel
Publication date
2015ISSN
0905-7161
Abstract
Objectives: The aim of this study was to determine the antibacterial properties of silver-doped titanium surfaces prepared with a novel electrochemical anodizing process. Material and methods: Titanium samples were anodized with a pulsed process in a solution of silver nitrate and sodium thiosulphate at room temperature with stirring. Samples were processed with different electrolyte concentrations and treatment cycles to improve silver deposition. Physicochemical properties were determined by X-ray photoelectron spectroscopy, contact angle measurements, white-light interferometry, and scanning electron microscopy. Cellular cytotoxicity in human fibroblasts was studied with lactate dehydrogenase assays. The in vitro effect of treated surfaces on two oral bacteria strains (Streptococcus sanguinis and Lactobacillus salivarius) was studied with viable bacterial adhesion measurements and growth curve assays. Nonparametric statistical Kruskal-Wallis and Mann-Whitney U-tests were used for multiple and paired comparisons, respectively. Post hoc Spearman's correlation tests were calculated to check the dependence between bacteria adhesion and surface properties. Results: X-ray photoelectron spectroscopy results confirmed the presence of silver on treated samples and showed that treatments with higher silver nitrate concentration and more cycles increased the silver deposition on titanium surface. No negative effects in fibroblast cell viability were detected and a significant reduction on bacterial adhesion in vitro was achieved in silver-treated samples compared with control titanium. Conclusions: Silver deposition on titanium with a novel electrochemical anodizing process produced surfaces with significant antibacterial properties in vitro without negative effects on cell viability.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
616.3 - Pathology of the digestive system. Complaints of the alimentary canal
Keywords
Titani
Materials biomèdics
Anodització
Antibacterià
Adhesió bacteriana
Deposició de plata
Deposició-química-vapor
Bacteris
Citotoxicitat in vitro
Implantació iònica
Implants dentals
Recobriments antibacterians
Staphylococcus aureus
Nanopartícules
Titanio
Materiales biomédicos
Anodización
Antibacterias
Adhesión bacteriana
Deposición de plata
Deposición-química-vapor
Bacterias
Citotoxicidad in vitro
Implantación iónica
Implantes dentales
Recubrimientos antibacterianos
Staphylococcus aureus
Nanopartículas
Titanium
Biomedical materials
Anodizing
Antibacterial
Bacterial adhesion
Silver deposit
Deposition-chemistry-steam
Bacteria
In vitro cytotoxicity
Ionic implantation
Dental implants
Antibacterial coatings
Staphylococcus aureus
Nanoparticles
Pages
29
Publisher
John Wiley & Sons
Collection
26;10
Is part of
Clinical Oral Implants Research
Citation
Godoy Gallardo, Maria; Rodríguez Hernández, Ana Guadalupe; Delgado, Luis M. [et al.]. Silver deposition on titanium surface by electrochemical anodizing process reduces bacterial adhesion of Streptococcus sanguinis and Lactobacillus salivarius. Clinical Oral Implants Research, 2014, 26(10), p. 1170-1179. Disponible en: <https://onlinelibrary.wiley.com/doi/10.1111/clr.12422>. Fecha de acceso: 12 nov. 2021. DOI: 10.1111/clr.12422
Grant agreement number
info:eu-repo/grantAgreement/MICINN/MAT2009-12547
This item appears in the following Collection(s)
- Odontologia [228]
Rights
© John Wiley & Sons, Inc.