Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study
Publication date
2021-09ISSN
2590-0064
Abstract
Biomimetic calcium-deficient hydroxyapatite (CDHA) as a bioactive material exhibits exceptional intrinsic osteoinductive and osteogenic properties because of its nanostructure and composition, which promote a favorable microenvironment. Its high reactivity has been hypothesized to play a relevant role in the in vivo performance, mediated by the interaction with the biological fluids, which is amplified by its high specific surface area. Paradoxically, this high reactivity is also behind the in vitro cytotoxicity of this material, especially pronounced in static conditions. The present work explores the structural and physicochemical changes that CDHA undergoes in contact with physiological fluids and to investigate its interaction with proteins. Calcium-deficient hydroxyapatite discs with different micro/nanostructures, coarse (C) and fine (F), were exposed to cell-free complete culture medium over extended periods of time: 1, 7, 14, 21, 28, and 50 days. Precipitate formation was not observed in any of the materials in contact with the physiological fluid, which would indicate that the ionic exchanges were linked to incorporation into the crystal structure of CDHA or in the hydrated layer. In fact, CDHA experienced a maturation process, with a progressive increase in crystallinity and the Ca/P ratio, accompanied by an uptake of Mg and a B-type carbonation process, with a gradual propagation into the core of the samples. However, the reactivity of biomimetic hydroxyapatite was highly dependent on the specific surface area and was amplified in nanosized needle-like crystal structures (F), whereas in coarse specimens the ionic exchanges were restricted to the surface, with low penetration in the material bulk. In addition to showing a higher protein adsorption on F substrates, the proteomics study revealed the existence of protein selectivity toward F or C microstructures, as well as the capability of CDHA, and more remarkably of F-CDHA, to concentrate specific proteins from the culture medium. Finally, a substantial improvement in the material's ability to support cell proliferation was observed after the CDHA maturation process.
Document Type
Article
Document version
Published version
Language
English
Subject (CDU)
62 - Engineering. Technology in general
620 - Materials testing. Commercial materials. Power stations. Economics of energy
Keywords
Fosfat de calci
Adsorció de proteïnes
Nanostructura
Intercanvi iònic
Espectroscòpia Raman
Fosfato de calcio
Absorción de proteínas
Nanoestructuras
Intercambio iónico
Espectroscopia Raman
Calcium phosphates
Protein adsorption
Nanostructure
Ion exchange
Raman spectroscopy
Pages
17
Publisher
Elsevier
Collection
12;
Is part of
Materials Today Bio
Citation
Konka, J.; Espanol, M.; Bosch Canals, Begoña Maria [et al.]. Maturation of biomimetic hydroxyapatite in physiological fluids: a physicochemical and proteomic study. Materials Today Bio, setembre 2021, 12, 100137. Disponible en: <https://www.sciencedirect.com/science/article/pii/S2590006421000454>. Fecha de acceso: 7 oct. 2021. DOI: 10.1016/j.mtbio.2021.100137
This item appears in the following Collection(s)
- Ciències Bàsiques [65]
Rights
© 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by-nc-nd/4.0/