Reconfigurable multiplexed point of care system for monitoring type 1 diabetes patients
Author
Márquez, Augusto
Aymerich, Joan
Dei, Michele
Rodríguez-Rodríguez, Rosalía
Vázquez-Carrera, Manuel
Pizarro-Delgado, Javier
Giménez-Gómez, Pablo
Merlos, Angel
Terés, Lluís
Publication date
2019-07ISSN
0956-5663
Abstract
At the point of care (POC), on-side clinical testing allows fast biomarkers determination even in resource-limited environments. Current POC systems rely on tests selective to a single analyte or complex multiplexed systems with important portability and performance limitations. Hence, there is a need for handheld POC devices enabling the detection of multiple analytes with accuracy and simplicity. Here we present a reconfigurable smartphone-interfaced electrochemical Lab-on-a-Chip (LoC) with two working electrodes for dual analyte determination enabling biomarkers' selection in situ and on-demand. Biomarkers selection was achieved by the use of electrodepositable alginate hydrogels. Alginate membranes containing either glucose oxidase (GOx) or lactate oxidase (LOx) were selectively electrodeposited on the surface of each working electrode in around 4 min, completing sample measurement in less than 1 min. Glucose and lactate determination was performed simultaneously and without cross-talk in buffer, fetal bovine serum (FBS) and whole blood samples, the latter being possible by the size-exclusion filtration capacity of the hydrogels. At optimal conditions, glucose and lactate were determined in a wide linear range (0–12 mM and 0–5 mM, respectively) and with high sensitivities (0.24 and 0.54 μA cm−2 mM−1, respectively), which allowed monitoring of Type-1 diabetic patients with a simple dual analysis system. After the measurement, membranes were removed by disaggregation with the calcium-chelator phosphate buffer. At this point, new membranes could be electrodeposited, this time being selective to the same or another analyte. This conferred the system with on-demand biomarkers’ selection capacity. The versatility and flexibility of the current architecture is expected to impact in POC analysis in applications ranging from homecare to sanitary emergencies.
Document Type
Article
Document version
Accepted version
Language
English
Subject (CDU)
61 - Medical sciences
Keywords
Diabetis
Elèctrodes
Mamífers
Marcadors bioquímics
Glucosa
Diabetes
Electrodos
Mamíferos
Marcadores bioquímicos
Glucosa
Diabetes
Electrodes
Mammal
Biochemical markers
Glucose
Pages
31
Publisher
Elsevier
Collection
136;
Is part of
Biosensors and Bioelectronics
Citation
Márquez, Augusto; Aymerich, Joan; Dei, Michele [et al.]. Reconfigurable multiplexed point of care system for monitoring type 1 diabetes patients. Biosensors and Bioelectronics, 2019, 136, p. 38-46. Disponible en: <https://www.sciencedirect.com/science/article/pii/S0956566319302945>. Fecha de acceso: 7 jul. 2020. DOI: 10.1016/j.bios.2019.04.015
Grant agreement number
info:eu-repo/grantAgreement/ES/1PE/TEC2014-54449-C3-1-R
This item appears in the following Collection(s)
- Ciències de la Salut [740]
Rights
© 2019 Elsevier B.V. All rights reserved.