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dc.contributor.authorBarneda-Zahonero, Bruna
dc.contributor.authorMiñano Molina, Alfredo Jesús
dc.contributor.authorBadiola, Nahuai
dc.contributor.authorFadó Andrés, Rut
dc.contributor.authorXifró Collsamata, Xavier
dc.contributor.authorSaura, Carlos A.
dc.contributor.authorRodríguez Álvarez, José
dc.date.accessioned2019-10-19T14:54:18Z
dc.date.available2019-10-19T14:54:18Z
dc.date.issued2009-12-15
dc.identifier.citationBarneda-Zahonero, B., Miñano-Molina, A., Badiola, N., Fadó, R., Xifró, X., Saura, C. A., & Rodríguez-Alvarez, J. (2009). «Bone morphogenetic protein-6 promotes cerebellar granule neurons survival by activation of the MEK/ERK/CREB pathway». Molecular biology of the cell, 20(24), 5051-5063ca
dc.identifier.issn1059-1524ca
dc.identifier.urihttp://hdl.handle.net/20.500.12328/1261
dc.description.abstractBone morphogenetic proteins (BMPs) have been implicated in the generation and postnatal differentiation of cerebellar granule cells (CGCs). Here, we examined the eventual role of BMPs on the survival of these neurons. Lack of depolarization causes CGC death by apoptosis in vivo, a phenomenon that is mimicked in vitro by deprivation of high potassium in cultured CGCs. We have found that BMP-6, but not BMP-7, is able to block low potassium–mediated apoptosis in CGCs. The neuroprotective effect of BMP-6 is not accompanied by an increase of Smad translocation to the nucleus, suggesting that the canonical pathway is not involved. By contrast, activation of the MEK/ERK/CREB pathway by BMP-6 is necessary for its neuroprotective effect, which involves inhibition of caspase activity and an increase in Bcl-2 protein levels. Other pathways involved in the regulation of CGC survival, such as the c-Jun terminal kinase and the phosphatidylinositol 3-kinase (PI3K)-Akt/PKB, were not affected by BMP-6. Moreover, failure of BMP-7 to activate the MEK/ERK/CREB pathway could explain its inability to protect CGCs from low potassium–mediated apoptosis. Thus, this study demonstrates that BMP-6 acting through the noncanonical MEK/ERK/CREB pathway plays a crucial role on CGC survival.ca
dc.format.extent13ca
dc.language.isoengca
dc.publisherMolecular biology of the cellca
dc.rightshttps://creativecommons.org/licenses/by/4.0/ca
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subject.otherBMPca
dc.subject.otherproteinca
dc.subject.otherproteinaca
dc.subject.otherCerebellarca
dc.subject.othercerebralca
dc.titleBone morphogenetic protein-6 promotes cerebellar granule neurons survival by activation of the MEK/ERK/CREB pathwayca
dc.typeinfo:eu-repo/semantics/articleca
dc.description.versioninfo:eu-repo/semantics/acceptedVersionca
dc.embargo.termscapca
dc.subject.udc616.8ca
dc.identifier.doihttps://doi.org/10.1091/mbc.e09-05-0424ca


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