Phosphoregulation of the oncogenic protein regulator of cytokinesis 1 (PRC1) by the atypical CDK16/CCNY complex
Autor/a
Hernández Ortega, Sara
Sánchez Botet, Abril
Quandt Herrera, Eva
Masip, Núria
Gasa Colom, Laura
Verde, Gaetano
Jiménez Jiménez, Javier
Levin, Rebecca
Rugatanira, Florentine U.
Burlingame, Alma L.
Wolfgeher, Donald
P.C. Ribeiro, Mariana
Kron, Stephen J.
M. Shokat, Kevan
Clotet Erra, Josep
Data de publicació
2019DOI
10.1038/s12276-019-0242-2
ISSN
1226-3613
Resum
CDK16 (also known as PCTAIRE1 or PCTK1) is an atypical member of the cyclin-dependent kinase (CDK) family that forms an active complex with cyclin Y (CCNY). Although both proteins have been recently implicated in cancer pathogenesis, it is still unclear how the CDK16/CCNY complex exerts its biological activity. To understand the CDK16/CCNY network, we used complementary proteomic approaches to identify potential substrates of this complex. We identified several candidates implicating the CDK16/CCNY complex in cytoskeletal dynamics, and we focused on the microtubule-associated protein regulator of cytokinesis (PRC1), an essential protein for cell division that organizes antiparallel microtubules and whose deregulation may drive genomic instability in cancer. Using analog-sensitive (AS) CDK16 generated by CRISPR-Cas9 mutagenesis in 293T cells, we found that specific inhibition of CDK16 induces PRC1 dephosphorylation at Thr481 and delocalization to the nucleus during interphase. The observation that CDK16 inhibition and PRC1 downregulation exhibit epistatic effects on cell viability confirms that these proteins can act through a single pathway. In conclusion, we identified PRC1 as the first substrate of the CDK16/CCNY complex and demonstrated that the proliferative function of CDK16 is mediated by PRC1 phosphorylation. As CDK16 is emerging as a critical node in cancer, our study reveals novel potential therapeutic targets.
Tipus de document
Article
Versió del document
Versió acceptada
Llengua
Anglès
Matèries (CDU)
616.3 - Patologia de l'aparell digestiu. Odontologia
Paraules clau
Phosphoregulation
Fosforegulació
Fosforegulación
cytokinesis 1
oncogenic protein regulator
regulador de proteïnes oncogènic
regulador de proteínas oncogénico
Pàgines
17
Publicat per
Experimental and molecular medicine
Citació
Hernández-Ortega, S., Sánchez-Botet, A., Quandt, E., Masip, N., Gasa, L., Verde, G., ... & Wolfgeher, D. (2019). Phosphoregulation of the oncogenic protein regulator of cytokinesis 1 (PRC1) by the atypical CDK16/CCNY complex. Experimental & molecular medicine, 51(4), 44.
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