• CDK-mediated Yku80 phosphorylation regulates the balance between non-homologous end joining (NHEJ) and homologous directed recombination (HDR) 

      Carballar, Reyes; Martínez-Láinez, Joan M.; Samper, Bàrbara; Bru, Samuel; Bállega, Elisabet; Mirallas, Oriol; Ricco, Natalia; Clotet, Josep; Jiménez, Javier (Journal of Molecular Biology, 2020)
      There are two major pathways for repairing DNA double-strand breaks (DSBs): homologous directed recombination (HDR) and non-homologous end-joining (NHEJ). While NHEJ functions throughout the cell cycle, ...
    • Comprehensive and quantitative analysis of G1 cyclins. A tool for studying the cell cycle 

      Ballega, Elisabet; Carballar Ruiz, María de los Reyes; Samper Martín, Bàrbara; Ricco Pacheco, Natalia; Ribeiro, Mariana P. C.; Bru Rullo, Samuel; Jiménez, Javier; Clotet Erra, Josep (PLoS One, 2019-06-25)
      In eukaryotes, the cell cycle is driven by the actions of several cyclin dependent kinases (CDKs) and an array of regulatory proteins called cyclins, due to the cyclical expression patterns of the latter. ...
    • Defective in mitotic arrest 1 (Dma1) ubiquitin ligase controls G1 cyclin degradation 

      Hernández-Ortega, Sara; Bru Rullo, Samuel; Ricco Pacheco, Natalia; Ramírez, Sara; Casals i Farré, Núria; Jiménez, Javier; Isasa, Marta; Crosas, Bernat; Clotet Erra, Josep (Journal of Biological Chemistry, 2013-02)
      Progression through the G1 phase of the cell cycle is controlled by diverse cyclin-dependent kinases (CDKs) that might be associated to numerous cyclin isoforms. Given such complexity, regulation of ...
    • Polyphosphate degradation by Nudt3-Zn2+ mediates oxidative stress response 

      Samper Martín, Bàrbara; Sarrias, Ana; Lázaro, Blanca; Pérez-Montero, Marta; Rodríguez-Rodríguez, Rosalía; P.C. Ribeiro, Mariana; Bañón, Aitor; Wolfgeher, Donald; Jessen, Henning J.; Alsina, Berta; Clotet Erra, Josep; Kron, Stephen J.; Saiardi, Adolfo; Jiménez, Javier; Bru Rullo, Samuel (Cell Reports, 2021)
      Polyphosphate (polyP) is a polymer of hundreds of phosphate residues present in all organisms. In mammals, polyP is involved in crucial physiological processes, including coagulation, inflammation, and ...
    • Protocol to quantify polyphosphate in human cell lines using a tagged PPBD peptide 

      Jiménez, Javier; Lázaro, Blanca; Sarrias, Ana; Tadeo, Francisco J.; Pérez-Montero, Marta; Clotet, Josep; Bru, Samuel (STAR Protocols, 2022)
      Polyphosphate (polyP) is an evolutionarily conserved polymer of phosphates that is difficult to study in human cells because of its low concentration and high lability. First, we described how to express ...